Emergomyces
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA747863 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Emergomyces orientalis strain:5z489 Genome sequencing and assembly
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Project description:Emergomyces orientalis emergomycosis (PRJCA044007)
| PRJDB39066 | ENA
Project description:Emergomyces africanus strain:CBS 136260 Genome sequencing and assembly
| PRJNA284519 | ENA
Project description:Whole genome sequencing of SYBARIS Aspergillus spp. known to be multi-drug resistant and difficult to treat. Aim of this experiment is to investigate the genetic basis of susceptibility to disease and elucidate molecular mechanisms of drug resistance in these strains.
2016-04-30 | E-SYBR-1 | biostudies-arrayexpress
Project description:We detected Emergomyces africanus, a thermally dimorphic fungus that causes an HIV-associated systemic mycosis, by PCR in 18 (30%) of 60 soil samples from a wide range of habitats in South Africa. Direct and indirect culture techniques were unsuccessful. Experimental intraperitoneal inoculation of conidia induced murine disease.
| S-EPMC5782882 | biostudies-literature
Project description:Emergomyces africanus is a thermally dimorphic fungus and one of the leading causes of emergomycosis, a neglected infection primarily affecting immunocompromised individuals. Despite its clinical relevance, how E. africanus adapts to the host environment remains unknown. Recent studies suggest that extracellular vesicles (EVs) may play a key role during host adaptation by modulating immune responses and transporting virulence factors. It remains unknown whether E. africanus produces EVs. Here, we demonstrated EV production in E. africanus and investigated their size and composition after cultivation in nutrient-rich and nutrient-limited media, mimicking environmental and host-like conditions. In addition, we evaluated the effect of E. africanus EVs released in nutrient-limited media on bone marrow-derived dendritic cells (BMDCs) and bone marrow-derived macrophages (BMDMs). Under nutrient limitation, E. africanus released EVs enriched in proteins that, in other models, are virulence-associated, including catalase, HSP60, and chitinase, whereas EVs from rich media carried proteins linked to anabolic pathways. The presence of -1,3-glucans and a higher content of chitin-like structures were also detected in EVs released in nutrient-limited conditions. EVs from nutrient-limited conditions activated BMDCs, increasing MHC-II and CD40 expression and promoting a pro-inflammatory cytokine profile (IL-6 and TNF-α). In contrast, BMDMs exhibited elevated IL-10 levels, suggesting an anti-inflammatory shift. Remarkably, EV pre-treatment enhanced BMDM antifungal activity, significantly reducing E. africanus viability post-infection. These findings show that E. africanus dynamically adjust their EVs cargo in response to environmental cues, directly influencing immune modulation and fungal survival. Indeed, pre-treatment of the insect Galleria mellonella with these EVs induced a protective response against a lethal inoculum of Histoplasma capsulatum. This study reinforces the potential of EVs as therapeutic targets and offers new insights into how E. africanus adapts to the host environment.
2026-02-05 | PXD062584 | Pride
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
| 2533916 | ecrin-mdr-crc
Project description:The following CGH experiments were conducted on six sectors (S1-S6) from a single primary ductal carcinoma tumor (T14) using the Sector-Ploidy-Profiling (SPP) Approach. SPP involves macro-dissecting the tumor, flow-sorting nuclei by differences in total genomic DNA content and profiling the genome of the tumor subpopulations.
2009-07-10 | GSE16611 | GEO
Project description:The following CGH experiments were conducted on six sectors (S1-S6) from a single primary ductal carcinoma tumor (T13) using the Sector-Ploidy-Profiling (SPP) Approach. SPP involves macro-dissecting the tumor, flow-sorting nuclei by differences in total genomic DNA content and profiling the genome of the tumor subpopulations.
2009-07-10 | GSE16610 | GEO