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HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure

ABSTRACT: HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure

PROVIDER: PRJNA749071 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15210200_1.fastq.gz	Fastqsanger.gz
	SRR15210200_2.fastq.gz	Fastqsanger.gz
	SRR15218300_1.fastq.gz	Fastqsanger.gz
	SRR15218300_2.fastq.gz	Fastqsanger.gz
	SRR15218400_1.fastq.gz	Fastqsanger.gz

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HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [ChIP-Seq]

Project description:HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [ChIP-Seq]

| PRJNA749075 | ENA

HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [Methyl-seq]

Project description:HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [Methyl-seq]

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HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [RNA-Seq]

Project description:HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [RNA-Seq]

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HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [RNA-Seq]

Project description:Recent advances in synthetic mammalian embryo models have opened new avenues to understand the complex events controlling lineage decisions and morphogenetic processes occurring during peri-implantation and early organogenesis. Two main strategies have been developed to build embryo-like structures (ELS): by assembling extraembryonic and embryonic stem cells (ESCs) or by subjecting ESCs to various morphogens. Here, we show that mouse ESCs solely exposed to chemical inhibition of SUMOylation, a posttranslational modification which acts as a general barrier to cell-fate transitions, generates early ELSs comprising both anterior neural and trunk-associated regions. HypoSUMOylated ESCs give rise to adherent spheroids which, once in suspension, self-organize into gastrulating structures containing cell types spatially and functionally related to embryonic and extraembryonic compartments. Alternatively, spheroids cultured in an optimized droplet-microfluidic device form elongated structures characterized by a multi-axial organization reminiscent of natural embryo morphogenesis. Single-cell RNA-sequencing further revealed a variety of cellular lineages including properly positioned anterior neuronal cell types, Schwann cell precursors and paraxial mesoderm segmented into somite-like structures. Mechanistically, transient SUMOylation suppression gradually increases DNA methylation genome-wide and repressive marks deposition at Nanog, enhancing ESC plasticity. Our approach provides a proof of principle for a potential new strategy to study early embryogenesis by targeting reprogramming roadblocks to shape multicellular architecture.

2023-03-17 | GSE180676 | GEO

HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [Methyl-seq]

2023-03-17 | GSE180597 | GEO

HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure [ChIP-Seq]

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HypoSUMOylation in embryonic stem cells generate head-trunk embryo-like structures [scRNAseq]

Project description:HypoSUMOylation in embryonic stem cells generate head-trunk embryo-like structures [scRNAseq]

| PRJNA749070 | ENA

HypoSUMOylation in embryonic stem cells generate head-trunk embryo-like structures [ChIP-seq]

Project description:HypoSUMOylation in embryonic stem cells generate head-trunk embryo-like structures [ChIP-seq]

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HypoSUMOylation in embryonic stem cells generates head-and-trunk embryo-like structure

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-17 | GSE180489 | GEO

Brief suppression of SUMOylation in embryonic stem cells generates embryo-like structures

Project description:Recent advances in synthetic mammalian embryo models have opened new avenues to understand the complex events controlling lineage specification and morphogenetic processes occurring during peri-implantation and early organogenesis. Two main strategies have been developed to build embryo-like structures (ELSs): by assembling extraembryonic and embryonic stem cells (ESCs) or by subjecting ESCs to various morphogens. Here, we show that mouse ESCs solely exposed to chemical inhibition of SUMOylation, a post-translational modification which acts as a general chromatin barrier to cell fate transitions, generates ELSs comprising both anterior neural and trunk-associated regions. HypoSUMOylation-instructed ESCs first give rise to adherent spheroids which, once in suspension, self-organize into gastrulating structures containing cell types spatially and functionally related to embryonic and extraembryonic compartments. Alternatively, spheroids cultured in an optimized droplet-microfluidic device form elongated structures that undergo axial organization reminiscent of natural embryo morphogenesis. Single-cell RNA-sequencing further revealed a variety of cellular lineages including properly positioned anterior neuronal cell types, Schwann cell precursors and paraxial mesoderm segmented into somite-like structures. Mechanistically, transient SUMOylation suppression gradually increases DNA methylation genome-wide and repressive marks deposition at Nanog, enhancing ESC plasticity. Our approach provides a proof of principle for a potential strategy to study early embryogenesis by targeting molecular roadblocks of cell fate change to shape multicellular architecture.

2023-04-13 | PXD031548 | Pride

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