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Human lung cancer cell line A549 +/- CD43 knockdown

ABSTRACT: Human lung cancer cell line A549 +/- CD43 knockdown

PROVIDER: PRJNA749458 | ENA |

REPOSITORIES: ENA

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Human lung cancer cell line A549 +/- CD43 knockdown

Project description:The human lung cancer cell line A549 with high CD43 expression was stably trasfected with a vector expressing a non-functional shRNA or 3 of these same vectors each expressing a different shRNA targeting human CD43 mRNA. The transcriptomes of these two daughter lines were then compared by differential microarray analysis. The generation and functional characteristics of these two daughter cell lines are described by Fu et al., in the International Journal of Cancer, volume 132, pages 1761-1770, published in 2013.

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Expression data from ORMDL3 knockdown in the A549 Human Lung Epithelial cell line

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Project description:Purpose of the study was to investigate the impact of reducing the efficiency of minor (U12-type) splicing on the growth and gene expression of a human cancer cell line. We chose the A549 cell line, derived from a human lung adenocarcinoma, for this study. There are only approximately 700 genes (3.5%) in the human genome that contain a minor intron. Most transcripts (96.5%) require only major (U2-dependent) splicing for correct gene expression. This is carried out by the major spliceosome. A different spliceosome, known as the minor or U11/12-type spliceosome, is required to excise minor introns from pre-RNA transcripts that contain them. One of the unique protein components of the minor spliceosome is a 65kDa protein encoded by the RNPC3 gene. To reduce the efficiency of minor splicing, we used siRNA knockdown methodology to deplete RNPC3 transcripts in A549 cells by approximately 70%. Specifically, A549 cells in growth phase were transfected with siRNA targeted to RNPC3 transcripts for 72h. Control A549 cells were treated in the same experiment with non-targeted (NT) siRNA molecules instead of siRNA targeted to RNPC3.

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