Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human Apoe3 and Apoe4 alleles have differential effects on mouse olfactory epithelium

ABSTRACT: Human Apoe3 and Apoe4 alleles have differential effects on mouse olfactory epithelium

PROVIDER: PRJNA750450 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15279832_1.fastq.gz	Fastqsanger.gz
	SRR15279832_2.fastq.gz	Fastqsanger.gz
	SRR15279833_1.fastq.gz	Fastqsanger.gz
	SRR15279833_2.fastq.gz	Fastqsanger.gz
	SRR15279834_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Human Apoe3 and Apoe4 alleles have differential effects on mouse olfactory epithelium

Project description:Alzheimer’s Disease (AD) is a progressive age-dependent disorder whose risk is determined in large part by genetic factors. We investigated the effects of human APOE3 and APOE4 alleles on the mouse olfactory epithelium.

2021-07-30 | GSE181038 | GEO

Differential gene expression in APOE3 and APOE4 gene targeted replacement mice

Project description:The aim of the study was to investigate hepatic gene expression profiles differentially regulated by the APOE genotype in gene targeted replacement mice. The APOE4 genotype is associated with increased mortality in the elderly and is an independent risk factor for age-dependent chronic diseases. However, little is known about the underlying mechanisms and molecular targets involved in the APOE4-risk association. As APOE is centrally involved in lipid and cholesterol metabolism and in large part is produced in the liver, we analyzed hepatic RNA profiles of APOE4- and APOE3-expressing mice. 2 groups of 5 animals with 1 liver extract per animal. Mice were homozygous for a human APOE3 or APOE4 gene targeted replacement of the endogenous mouse Apoe gene (B6.129P2-Apoetm2(APOE*3)Mae N8 or B6.129P2-Apoetm3(APOE*4)Mae N8, Taconic Transgenic ModelsM-bM-^DM-", http://www.taconic.com/wmspage.cfm?parm1=2542), purchased at the age of 6-8 weeks, strain C57BL/6, 3 months old at the performance of the microarray, 6 weeks on a high-fat diet containing 41% energy from milk fat and 2 g/kg cholesterol.

2012-12-15 | E-GEOD-42930 | biostudies-arrayexpress

LC-MS/MS of HNE-apoE3 and HNE-apoE4

Project description:The overall goal of our study is to understand the effect of HNE modification on the structure and function of apolipoprotein E3 (apoE3) and apoE4, which play a critical role in brain cholesterol homeostasis. Immunoblots indicated HNE modification of recombinant apoE3 and apoE4 with a major band at ~36 kDa, while LC-MS/MS analysis revealed modification of H140 and H299 in both, and additionally at C112 in apoE3.

2024-08-09 | PXD038105 | Pride

Proteomics of APOE3 and APOE4 BMDMs

Project description:The protein expression differences of APOE3 and APOE4 bone marrow-derived macrophages.

2025-01-10 | PXD059706 |

Differential gene expression in APOE3 and APOE4 gene targeted replacement mice

2012-12-15 | GSE42930 | GEO

Human APOE3 expressing mice are more prone to develop fatty liver disease on a high fat and sugar diet compared to APOE4 mice

Project description:Recent findings suggest that the human APOE epsilon 4 allele protects against non-alcoholic fatty liver disease, while APOE epsilon 3 promotes hepatic steatosis and steatohepatitis. We performed an untargeted proteome analysis of the liver and identified a great number of proteins differently expressed in obese APOE3 and APOE4 mice. The majority of the proteins up-regulated in APOE3 can be grouped to inflammation and damage-associated response, cytoskeleton and lipid storage. In contrast, those proteins that are up-regulated in APOE4 can be related to intermediate filament modifications, biotransformation and amino acid metabolism. Results of the targeted quantitative RT-PCR and Western blot experiments contribute to the overall finding that APOE3 promotes hepatic steatosis, inflammatory- and damage-associated response signaling and fibrosis in the liver of obese mice. One of the proteins that were up-regulated in obese as well as lean APOE4 compared to APOE3 mice is parvulin 14 (Pin4). Up-regulation of parvulin 14 may be involved in the protection against fatty liver disease evident in the presence of APOE4.

2024-05-21 | PXD033973 | Pride

Mouse Olfactory Epithelium

Project description:Mouse Olfactory Epithelium

| PRJNA660059 | ENA

Choroid Plexus Gene Expression in ApoE3 or ApoE4-Knockin Mouse Choroid Plexuses

Project description:Laser capture microdissected choroid plexuses were obtained and expression arrays were generated to investigate gene expression in ApoE-Knockin choroid plexuses; the choroid plexus forms the cerebrospinal fluid, the cerebrospinal fliod barrier, functions as the major gateway for blood-born leukocytes to enter the brain in degenerative and inflammatory brain diseases, and the principal neuroimmune interface in the brain. We found lipid deposits in the aged choroid plexus of hyperlipidemic ApoE4-Knockin mice but none in normolipidemic ApoE4-Knockin or normolipidemic or hyperlipidemic ApoE3-Knockin control choroid plexuses. Here, we studied the functional impact and gene epressions these choroid plexuses.

2018-11-20 | GSE85774 | GEO

The human ApoE4 variant reduces functional recovery and neuronal sprouting after incomplete spinal injury in male mice

Project description:Patients carrying one or two ApoE4 alleles suffer from worse functional recovery after spinal cord injury. Using transgenic mice expression human ApoE3 or ApoE4 we investigated potential cellular mechanisms of reduced recovery after spinal cord injury. Bulk RNA sequencing of the spinal cord lesion site followed by pathway enrichment analysis predicts that ApoE4 mice have a higher inflammatory and extracellular matrix remodeling activity 7 days after spinal cord injury. Contrary, higher activities for neuronal projection and action potential patways were predicted in the ApoE3 mice at 21 days after injury.

2021-02-05 | GSE164688 | GEO

Mouse Olfactory Epithelium, whole olfactory mucosa, mouse

Project description:Mouse Olfactory Epithelium, whole olfactory mucosa, mouse

| PRJNA660060 | ENA