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PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation [RNA-seq]

ABSTRACT: PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation [RNA-seq]

PROVIDER: PRJNA751101 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17657871.fastq.gz	Fastqsanger.gz
	SRR17657872.fastq.gz	Fastqsanger.gz
	SRR17657873.fastq.gz	Fastqsanger.gz
	SRR17657874.fastq.gz	Fastqsanger.gz
	SRR17657875.fastq.gz	Fastqsanger.gz

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PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation.

Project description:PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation.

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PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation [ChIP-seq]

Project description:PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation [ChIP-seq]

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PRC2 shields naïve human pluripotent cells from trophectoderm and mesoderm differentiation [scRNA-seq]

Project description:Single-cell RNA-seq reveals that Polycomb repressive complex 2 (PRC2) maintains naïve pluripotency and restricts an intrinsic capacity of pre-implantation pluripotent stem cells to give rise to trophectoderm and mesoderm lineages. Inhibition of PRC2 forces naïve hESC into an ‘activated’ state through which differentiation into either trophectoderm or mesoderm lineages is triggered. This trajectory is distinct from embryonic lineage specification out of the post-implantation pluripotent state, hence PRC2-mediated repression provides a highly adaptive mechanism to restrict lineage potential during early human development.

2022-03-14 | GSE196280 | GEO

PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation [RNA-seq]

Project description:The first lineage choice made in human embryo development separates trophectoderm from the inner cell mass, which proceeds to form the pluripotent epiblast and primitive endoderm. Trophectoderm on the other hand gives rise to the placenta. Naïve pluripotent stem cells are derived from the pluripotent epiblast of the blastocyst and offer possibilities to explore how lineage integrity is maintained. Here, we discover that Polycomb repressive complex 2 (PRC2) restricts an intrinsic capacity of naïve pluripotent stem cells to give rise to trophectoderm. Through quantitative epigenome profiling, we find that broad histone H3 lysine 27 trimethylation (H3K27me3) hypermethylation is a common feature of naïve pluripotency across species. We define a previously unappreciated, naïve-specific set of bivalent promoters, featuring PRC2-mediated H3K27me3 concomitant with H3K4me3. Naïve bivalency maintains key trophectoderm transcription factors in a transcriptionally poised state that is resolved to an active state upon depletion of H3K27me3 via inhibition of the enzymatic subunits of PRC2, EZH1/2. Conversely, primed human embryonic stem cells cannot be driven towards trophectoderm development via PRC2 inhibition. While naïve and primed hESCs share the majority of bivalent promoters, PRC2 contributes to the repression of largely non-overlapping subsets of these promoters in each state, hence H3K27me3-mediated repression provides a highly adaptive mechanism to restrict lineage potential during early human development.

2021-08-23 | GSE181242 | GEO

PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation [ChIP-seq]

2021-08-23 | GSE181241 | GEO

PRC2 shields naïve human pluripotent cells from trophectoderm and mesoderm differentiation [scRNA-seq]

Project description:PRC2 shields naïve human pluripotent cells from trophectoderm and mesoderm differentiation [scRNA-seq]

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PRC2 shields naive pluripotent cells from trophectoderm and mesoderm differentiation.

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-08-23 | GSE181244 | GEO

α-Ketoglutarate promotes trophectoderm induction and maturation from naive human embryonic stem cells [RNA-Seq]

Project description:Development and lineage choice are driven by interconnected transcriptional, epigenetic and metabolic changes. Specific metabolites, such as α-ketoglutarate (αKG), function as signalling molecules affecting the activity of chromatin-modifying enzymes. However, how metabolism coordinates cell-state changes, especially in human pre-implantation development, remains unclear. Here we uncover that inducing naive human embryonic stem cells towards the trophectoderm lineage results in considerable metabolic rewiring, characterized by αKG accumulation. Elevated αKG levels potentiate the capacity of naive embryonic stem cells to specify towards the trophectoderm lineage. Moreover, increased αKG levels promote blastoid polarization and trophectoderm maturation. αKG supplementation does not affect global histone methylation levels; rather, it decreases acetyl-CoA availability, reduces histone acetyltransferase activity and weakens the pluripotency network. We propose that metabolism functions as a positive feedback loop aiding in trophectoderm fate induction and maturation, highlighting that global metabolic rewiring can promote specificity in cell fate decisions through intricate regulation of signalling and chromatin.

2025-02-27 | GSE247631 | GEO

α-Ketoglutarate promotes trophectoderm induction and maturation from naive human embryonic stem cells [ATAC-Seq]

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α-Ketoglutarate promotes trophectoderm induction and maturation from naive human embryonic stem cells [scRNA-Seq 3D]

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