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ABSTRACT: RNA sequence in pectoralis major and soleus of 7 week old Xinghua chicken

PROVIDER: PRJNA751251 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15315700.fastq.gz	Fastqsanger.gz
	SRR15315701.fastq.gz	Fastqsanger.gz
	SRR15315686.fastq.gz	Fastqsanger.gz
	SRR15315687.fastq.gz	Fastqsanger.gz
	SRR15315688.fastq.gz	Fastqsanger.gz

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Gallus gallus

Project description:ATAC-seq in pectoralis major and soleus of 7 week old Xinghua chicken

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Genome-wide transcriptome analysis of chickens during dietary methionine restriction reveals compromised immune competency

Project description:The goal of this study was to compare next-generation sequencing (NGS)-derived liver and pectoralis major transcriptome profilings (RNA-seq) of chicken fed control diet to treatment birds fed methionine restriction diet. We sequenced mRNA from 10 Gallus gallus males fed either control (L-Methionine) or L-Methionine-deficient diet (5 control and 5 treatment) from 3-5 weeks of age. We sequenced both liver and the pectoralis major muscle.

2017-04-21 | GSE98039 | GEO

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Project description:Expression data from chicken Pectoralis major

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Comparative transcriptome analysis reveals critical genes/pathways related yolk precursor synthesis in livers of Guangxi Ma chicken with different egg laying rate

Project description:Liver tissues of Guangxi Ma chicken from 32-week old with m performance, 50-week old with high and low laying performance, and 72-week old with high and low laying performance were collected and sequenced in quadruplicate using RNA-seq. The sequences were double-ended sequenced on the DNBSEQ sequencing platform. The sequence reads were quality controlled and then aligned with genomic sequences using HISAT2 program, quantified by featureCounts program, and gene expression levels were verified by qRT-PCR with SYBR Green detection. The results will be helpful to explore the factors that affecting laying performance from the perspective of yolk synthesis and provide a theoretical basis for improving the egg production of Guangxi Ma chicken.

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Deep Sequencing Analysis of miRNAs Expression in leg muscle of Xinghua Chicken

Project description:Purpose:We have used RNA-seq to examine of differentially expressed miRNAs in chicken leg muscle of three different development stages (11 embryo ages, 16 embryo ages, and 1 day old post hatch chick).The aims of this study are characterization of miRNAs differentially expressed in different developmental stage of chicken embryo, using RNA sequence sample. Methods: On this study we used two embryonic stage and one post hatch chick leg muscle of Xinghua chicken breed. Total RNA from E11 day embryo, E16 day embryo and 1 day post hatch chick was isolated by TRIzol following the manufacturer’s protocol (Invitrogen, CA, USA). Each stages were designed two samples, and the total samples were six (three group × two sample/group) and RNA samples of six individuals were pooled with equal amounts, and then were subjected to Illumina deep sequencing. Results: After eliminating adaptor and low-quality reads, a total of 5,302,700, 6,556,747, 5,359,793, 4,213,112, 7,112,885 and 7,469,939 clean reads were obtained in group E11 (E11.1-E11.2), group E16 (E16.1-E16.2) and group P1 (P1.1–P1.2) libraries, respectively. The clean reads were aligned to the chicken genome databases, miRBase, Rfam, RepBase and mRNA. Conclusions:To assess miRNA expression during chicken embryo skeletal muscle development, we sequenced and analyzed leg muscle at 11 day embryo age, 16 day embryo age, and 1 days post hatch.

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Project description:Spatial Transcriptomics of chicken pectoralis major muscle

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Project description:mRNA transcriptome sequencing of pectoralis and abdominal fat tissues of 43-week-old Gushi chicken

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Divergent Gene Expression through PI3K/Akt Signaling Pathway Cause Different Models of Hypertrophy Growth in Chicken

Project description:RNA-Seq technology was used to investigate differences in the gene expression of pectoralis muscle tissue between two chicken breeds (Ross as commercial (rapidly growing) and Isfahani as Iranian local breed (slow-growing)).

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Identification of genes regulated by long noncoding RNA H19 in skeletal muscle

Project description:Soleus muscle has the most abundant H19 expression compared to other skeletal muscle tissues. In order to identify genes regulated by long noncoding RNA H19 in skeletal muscle, we performed RNA-Seq with dissected WT and H19KO soleus muscles from 21 week old mice. Among the differentially expressed genes, we found skeletal muscle - overexpressed gene DUSP27, which potentially plays an important role in regulating skeletal muscle glucose metabolisim by regulating the activitiy of AMPK, might be a target of H19 mediated regulation.

2018-08-30 | GSE103202 | GEO

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