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MYC assembles and stimulates topoisomerases 1 & 2 in a “topisome” [CAD-seq]

ABSTRACT: MYC assembles and stimulates topoisomerases 1 & 2 in a “topisome” [CAD-seq]

PROVIDER: PRJNA752096 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15343162.fastq.gz	Fastqsanger.gz
	SRR15343163.fastq.gz	Fastqsanger.gz
	SRR15343164.fastq.gz	Fastqsanger.gz
	SRR15343165.fastq.gz	Fastqsanger.gz

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Similar Datasets

MYC assembles and stimulates topoisomerases 1 & 2 in a “topisome” [cross-CAD-seq]

Project description:MYC assembles and stimulates topoisomerases 1 & 2 in a “topisome” [cross-CAD-seq]

| PRJNA752095 | ENA

MYC assembles and stimulates topoisomerases 1 & 2 in a “topisome”

Project description:MYC assembles and stimulates topoisomerases 1 & 2 in a “topisome”

| PRJNA752089 | ENA

MYC assembles and stimulates topoisomerases 1 & 2 in a “topisome” [CAD-seq]

Project description:High-intensity transcription and replication supercoil DNA to levels that can impede or halt these processes. As a potent transcription amplifier and replication accelerator, the proto-oncogene MYC must somehow manage these high levels of torsional stress. By comparing gene expression with the recruitment of topoisomerases and MYC to promoters, we surmised a direct association of MYC with Topoisomerases 1 (TOP1) and 2A (TOP2A) that was confirmed in vitro and in vivo. Beyond recruiting topoisomerases, MYC directly stimulates their activities in vitro and in vivo. We identify a MYC-nucleated “topoisome” complex that unites and increases the levels of both both TOP1 and TOP2, as well as their activities at promoters and enhancers. Whether TOP2A or TOP2B is included in the topoisome, is dictated by the presence of c-MYC or MYCN, respectively. Thus, in vivo and in vitro, MYC assembles tools that simplify DNA topology and promote genome function under high output conditions.

2021-12-09 | GSE181448 | GEO

MYC assembles and stimulates topoisomerases 1 & 2 in a “topisome” [cross-CAD-seq]

2021-12-09 | GSE181449 | GEO

MYC assembles and stimulates topoisomerases 1 & 2 in a “topisome” [ChIP-seq]

Project description:MYC assembles and stimulates topoisomerases 1 & 2 in a “topisome” [ChIP-seq]

| PRJNA752097 | ENA

MYC assembles and stimulates topoisomerases 1 & 2 in a “topisome” [ChIP-seq]

2021-12-09 | GSE181447 | GEO

Project description:MYC assembles and stimulates topoisomerases 1 & 2 in a "topisome"

| PRJNA667145 | ENA

MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome".

Project description: Not available

| S-EPMC8750365 | biostudies-literature

MYC assembles and stimulates topoisomerases 1 & 2 in a “topisome”

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-12-09 | GSE181450 | GEO

MYC modulates TOP2A diffusion to promote substrate detection and activity [CAD-seq]

Project description:Topoisomerases are essential enzymes that alleviate DNA supercoiling by cleaving and resealing DNA strands. Regulation of their abundance and activity is crucial for maintaining genomic stability and supporting nuclear functions. Previously, we showed that the oncoprotein MYC recruits and stimulates topoisomerases to remove DNA entanglements generated by oncogenic transcription. Understanding this mechanism may suggest methods to inhibit MYC-driven topoisomerase activation, thereby selectively targeting tumor-specific transcription. Here, we demonstrate that the essential topoisomerase TOP2A in human cells exists in a dynamic equilibrium between sequestration in the nucleolus, substrate searching in transcription hubs, and active engagement on chromatin. This equilibrium is highly responsive to changes in DNA topology, allowing cells to regulate TOP2A levels in the nucleoplasm. Using single molecule tracking, we show that MYC accelerates TOP2A diffusion in cells. In vitro, we demonstrate that MYC decreases the size of TOP2A condensates, likely by limiting non-specific self interactions. By increasing TOP2A diffusion, MYC promotes substrate binding and increases TOP2A engagement on the chromatin genome-wide, revealing the mechanism underlying MYC stimulation of TOP2A activity.

2026-01-26 | GSE298118 | GEO

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