Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Genome-wide CRISPR screening

PROVIDER: PRJNA752869 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15371430_1.fastq.gz	Fastqsanger.gz
	SRR15371430_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

CRISPR-StAR, a paradigm leveraging internal controls, empowers genetic screening in vivo (batch 2)

Project description:Pooled genetic screening with CRISPR/Cas9 has enabled genome-wide high-resolution assignment of genes to phenotypes. To assess the effect of a given genetic perturbation, each sgRNA must be evaluated in hundreds of cells to overcome stochastic genetic drift and obtain robust results. In complex models that display particularly high heterogeneity, such as organoids or tumors transplanted into mice however, sufficient representation typically requires unreasonable scaling, thus preventing genome-wide screens at high resolution. Here we present CRISPR-StAR, a screening paradigm that overcomes intrinsic and extrinsic heterogeneity as well as genetic drift in bottlenecks by leveraging internal controls generated through activating sgRNAs only in half of the progenies of each cell. We use CRISPR-StAR to reveal in vivo-specific genetic dependencies in a genome-wide screen in mouse melanoma. Benchmarking to a conventional screening setup highlights the improved data quality this technology delivers. We anticipate CRISPR-StAR to set a new standard for genetic screening in complex models, foremost in vivo.

2024-11-05 | GSE262308 | GEO

CRISPR-StAR, a paradigm leveraging internal controls, empowers genetic screening in vivo (batch 1)

2024-11-05 | GSE262307 | GEO

RNA-seq and genome-wide CRISPR activation screening

Project description:RNA-seq and genome-wide CRISPR activation screening

| PRJNA917326 | ENA

Genotyping of C.elegans mutants - CRISPR/Cas9 of all GPCR and neuropeptide genes

Project description:CRISPR/Cas9 genome editing was used to disrupt nearly all the GPCR and neuropeptide genes from C. elegans genome. Multiple genes were disrupted in each strain for the purpose of screening. The genotype is the list of targeted genes

2023-06-02 | E-MTAB-12983 | biostudies-arrayexpress

Genome-wide CRISPR screening combined with ATAC-see assay

Project description:Genome-wide CRISPR screening combined with ATAC-see assay

| PRJNA603696 | ENA

The ER stress sensor IRE1 interacts with STIM1 to promote store-operated calcium entry, T cell activation, and muscular differentiation

Project description:CAMPARI2 CRISPR screening for SOCE modulators of ER stress. PC cells were sorted and sequenced for CRISPR whole KO library (De brie). Unsorted, SOrte din LOW PC and LOW PC Tunica treated fro 4hours were analysed.

2023-12-09 | E-MTAB-13548 | biostudies-arrayexpress

Genome-wide CRISPR screening using cell lines that harbor the mutant hTERT-CRISPR allele.

Project description:To assess the biological significances of p-hTERT in ALT cells, we used the CRISPR-Cas9 system targeting the hTERT gene and performed genome-wide CRISPR screening, which identified genes in the Fanconi anemia/BRCA pathway as synthetic lethal partners of hTERT.

2024-04-08 | GSE226994 | GEO

Genome-wide CRISPR screening reveals nucleotide synthesis negatively regulates autophagy

Project description:Genome-wide CRISPR screening reveals nucleotide synthesis negatively regulates autophagy

| PRJNA691936 | ENA

Project description:DNA-seq of genome-wide CRISPR screening in Bombyx mori

| PRJNA602216 | ENA

Project description:Wide-genome CRISPR screening with cetuximab using HNSCC cell line

| PRJNA1372793 | ENA