Ontology highlight
ABSTRACT:
PROVIDER: PRJNA753116 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:ChIP-seq experiment of transcription factor NFAT in Asian citrus psyllid Diaphorina citri. This experiment will figure out what gene was transcripted by NFAT in Diaphorina citri
2025-12-24 | GSE314527 | GEO
Project description:Paurocephala sauteri Enderlein, one of the most important pests of mulberry trees in the tropical and subtropical regions, the nymphs and adults of this species penetrate the veins on the back of leaves and buds of mulberry trees, nymph and female adult can secrete wax, these wax accumulated on the mulberry leaves significantly effect photosynthesis of leaves and silkworm cultivation. Therefore, this study aimed to clearly understand the wax-secreting system and characteristics of wax secretion, and see if there is any possible strategy to control the species population from angle of the wax-secreting process. The main findings were as follow: scanning electron microscopy revealed that the nymphs and female adults secreted wax in the form of long tubes with spherical ends, male adults only secreted honeydew. Additionally, the wax-secreting system of the nymphs and female adults were different, the nymph owned a bending moon-shaped wax gland, the first instar nymph wax gland was consisted of one circle of rectangular and depressed cells, but second to fifth instar nymph wax gland was made of one circle of rectangular and depressed cells, and one circle of round depressed cells. There were a certain number of white pores inside of these depressed cells. Wax filaments produced by these pores converted the into dense, tight and hollow wax tube. Gas chromatography/mass spectrometry showed that the main constituents of P. sauteri wax were hydrocarbons. Wax tube as a water conservation pool during the P. sauteri developmental process, this physiological feature may as a key point for a potential strategy to control P. sauteri, even other secreting insects.
2024-11-04 | MTBLS11229 | MetaboLights
Project description:Imidacloprid treated Diaphorina citri adults
| PRJNA579841 | ENA
Project description:We designed and constructed a genome-wide microarray with 22,987 70-mer oligonucleotides covering the presently known and predicted genes in the silkworm genome, and surveyed the gene expression in multiple silkworm tissues on day 3 of the fifth instar. Clusters of tissue-prevalent and tissue-specific genes and genes that are differentially expressed in different tissues were identified, and they reflect well major tissue-specific functions on the molecular level. The data presented in this study provide a new resource for annotating the silkworm genome. In the present study, we surveyed gene expression in the A/MSG, the PSG, testis, ovary, fat body, midgut, integument, hemocyte, malpighian tubule, and head from silkworm individuals on day 3 of the fifth instar. In order to establish gene expression differences between sexes, we prepared male and female samples of the same tissue. In addition, we also selectively performed the biological replicates at least twice for five tissues including testis, ovary, A/MSG, PSG and malpighian tubule, to evaluate biological reproducibility. In all, we prepared 30 two-channel hybridizations across the selected tissues for study. We extracted the single channel intensity instead of the ratio value from each two-channel hybridization for further analysis, a strategy that has been reported as being more flexible and valid previously.
2009-08-28 | E-GEOD-17571 | biostudies-arrayexpress
Project description:Beta-cypermethrin treated Diaphorina citri adults
| PRJNA579842 | ENA
Project description:Hemolymph was characterized from Diaphorina citri adults infected with the phytopathogen, Candidatus Liberibacter asiaticus (CLas) and compared with that from uninfected psyllids. This study identified 5531 and 3220 peptides within infected and uninfected hemolymph using nano LC-MS/MS. A reduced number of proteins were detected for D. citri and all known endosymbionts within infected hemolymph as compared to uninfected hemolymph. A large number of immune defense proteins were absent from D. citri hemolymph; however, a single recognition protein (PGRP), two serine protease inhibitors, three prophenoloxidase (proPO) enzymes, and a single serine protease in an uninfected D. citri were detected. The hemolymph is nearly devoid of nutrient storage proteins. This is the first proteomic analysis of D. citri hemolymph that also analyzes the components contributed by all of the endosymbionts. By comparing the contribution of each endosymbiont (CCR, CPA,WB) in the presence and absence of CLas infection, this study provides initial insights regarding the hemolymph response to microbial community shifts associated with D. citri infection status. Our data also present potential protein targets for analysis and disruption of CLas transmission that may facilitate management of huanglongbing (HLB) caused by CLas in citrus.
2017-01-18 | PXD005404 | Pride