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Quantitative Analysis of CXCL4 and TLR8 signaling crosstalk in human primary monocytes by RNA-Seq

ABSTRACT: Quantitative Analysis of CXCL4 and TLR8 signaling crosstalk in human primary monocytes by RNA-Seq

PROVIDER: PRJNA753814 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15415022.fastq.gz	Fastqsanger.gz
	SRR15415023.fastq.gz	Fastqsanger.gz
	SRR15415024.fastq.gz	Fastqsanger.gz
	SRR15415025.fastq.gz	Fastqsanger.gz
	SRR15415026.fastq.gz	Fastqsanger.gz

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Quantitative Analysis of CXCL4 and TLR8 signaling crosstalk in human primary monocytes by RNA-Seq

Project description:To gain a comprehensive understanding of gene regulation in CXCL4 and TLR8 signaling crosstalk, we treated primary human blood monocytes with CXCL4 and TLR8 ssRNA ligand ORN8L for 6 h and performed transcriptomic analysis via RNA-seq. We observed that CXCL4 interacted with TLR8 ssRNA ligand and triggered inflammatory cytokine storm including IL6, IL12p40, TNF and IFNβ, and pro-fibrotic gene expression and activated NLRP3 inflammasome leading to interleukin-1β (IL-1β) secretion and pyroptosis in human blood monocytes.

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