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N6-adenomethylation of GsdmC is essential for Lgr5+ stem cell survival and normal colon epithelial morphogenesis

ABSTRACT: N6-adenomethylation of GsdmC is essential for Lgr5+ stem cell survival and normal colon epithelial morphogenesis

PROVIDER: PRJNA753870 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15418941.fastq.gz	Fastqsanger.gz
	SRR15418942.fastq.gz	Fastqsanger.gz
	SRR15418982.fastq.gz	Fastqsanger.gz
	SRR15418983.fastq.gz	Fastqsanger.gz
	SRR15418984.fastq.gz	Fastqsanger.gz

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N6-adenomethylation of GsdmC is essential for Lgr5+ stem cell survival and normal colon epithelial morphogenesis [scRNA-seq]

Project description:N6-adenomethylation of GsdmC is essential for Lgr5+ stem cell survival and normal colon epithelial morphogenesis [scRNA-seq]

| PRJNA753949 | ENA

N6-adenomethylation of GsdmC is essential for Lgr5+ stem cell survival and normal colon epithelial morphogenesis [bulk RNA-seq]

Project description:N6-adenomethylation of GsdmC is essential for Lgr5+ stem cell survival and normal colon epithelial morphogenesis [bulk RNA-seq]

| PRJNA753948 | ENA

N6-adenomethylation of GsdmC is essential for Lgr5+ stem cell survival and normal colon epithelial morphogenesis [scRNA-seq]

Project description:The study explores the role of RNA methylation in the regulation of gut epithelial morphogenesis.

2021-09-30 | GSE181928 | GEO

N6-adenomethylation of GsdmC is essential for Lgr5+ stem cell survival and normal colon epithelial morphogenesis [bulk RNA-seq]

Project description:The study explores the role of RNA methylation in the regulation of gut epithelial morphogenesis.

2021-09-30 | GSE181927 | GEO

N6-adenomethylation of GsdmC is essential for Lgr5+ stem cell survival and normal colon epithelial morphogenesis

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-09-30 | GSE181929 | GEO

Effect of Gsdmc knockdown in murine KPC cells transcriptome

Project description:We identified that Gsdmc gene is important for pancreatic cancer in tumorigenesis and metastasis. Here we used KPC mice (LSL-KrasG12D/+; p53f/f; Pdx1-Cre)-derived cancer cells, KPC cells in short, to check the effects of Gsdmc knockdown on primary tumor growth and metastasis. The results suggested that Gsdmc knockdown inhibited the expression of key genes in multiple pathways, including cell migration, epithelial-mesenchymal transition, immune cell recruitment, and so on. The data deposited here provides the transcriptomic alteration of KPC cells after Gsdmc knockdown with two shRNAs.

2024-09-18 | GSE250062 | GEO

N<sup>6</sup>-adenomethylation of GsdmC is essential for Lgr5<sup>+</sup> stem cell survival to maintain normal colonic epithelial morphogenesis.

Project description:Gut epithelial morphogenesis is maintained by intestinal stem cells. Here, we report that depletion of N6-adenosine methyltransferase subunit Mettl14 from gut epithelial cells in mice impaired colon mucosal morphogenesis, leading to increased mucosal permeability, severe inflammation, growth retardation, and premature death. Mettl14 ablation triggered apoptosis that depleted Lgr5+ stem cells and disrupted colonic organoid growth and differentiation, whereas the inhibition of apoptosis rescued Mettl14-deleted mice and organoids. Mettl14 depletion disrupted N6-adenomethylation on GsdmC transcripts and abolished GsdmC expression. Reconstitution of Mettl14-deleted organoids or mice with GSDMC rescued Lgr5 expression and prevented apoptosis and mouse premature death, whereas GSDMC silence eliminated LGR5 and triggered apoptosis in human colonic organoids and epithelial cells. Mechanistically, Mettl14 depletion eliminated mitochondrial GsdmC, disrupted mitochondrial membrane potential, and triggered cytochrome c release that activates the pro-apoptotic pathway. In conclusion, GsdmC N6-adenomethylation protects mitochondrial homeostasis and is essential for Lgr5+ cell survival to maintain normal colonic epithelial regeneration.

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Nur77 induces GSDMC-mediated pyroptosis

Project description:Nur77 induces GSDMC-mediated pyroptosis

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Nur77 induces GSDMC-mediated pyroptosis

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2025-07-07 | GSE273365 | GEO

Transcriptomic profilling of Mex3a+ cells in colon adenomas compared to LGR5

Project description:Transcriptomic data related to 4 different subpopulations found in Mex3a Ki/+ Lgr5 Gfp in APCflfl adenomas in untreated animals. Adenomas where induced with 3%DSS and a single shot of 8mg/kg of Tamoxifen. The populations are refered as Mex3a + Lgr5, Mex3a- Lgr5+ Mex3a+ Lgr5- and Mex3a- Lgr5- according to the flow cytometry profile. Cells were isolated using FACsARIA (BD)

2022-05-12 | GSE163171 | GEO

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