Ontology highlight
ABSTRACT:
PROVIDER: PRJNA756307 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR15698716_subreads.fastq.gz | Fastqsanger.gz | |||
| SRR15698717_1.fastq.gz | Fastqsanger.gz | |||
| SRR15698717_2.fastq.gz | Fastqsanger.gz | |||
| SRR20755200_1.fastq.gz | Fastqsanger.gz |
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Project description:Coumaphos is an acaricide that is widely used to control Varroa mites in bee colonies. However, the extensive use of coumaphos against Varroa has resulted in the development of coumaphos resistant Varroa populations. In this study, the whole genome gene expression profile of 1) a coumaphos resistant V. destructor population (AN-CR) or 2) a coumaphos resistant V. destructor population pretreated with coumaphos (tAN-CR), was compared to the gene expression profile of a susceptible V. destructor population (ATH-S) using Illumina RNAseq.
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Project description:The mite Varroa destructor is currently the greatest threat to apiculture as it is causing a global decrease in honey bee colonies. However, it rarely causes serious damage to its native hosts, the eastern honey bees Apis cerana. To better understand the mechanism of resistance of A. cerana against the V. destructor mite, we profiled the metabolic changes that occur in the honey bee brain during V. destructor infestation. Brain samples were collected from infested and control honey bees and then measured using an untargeted liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based global metabolomics method, in which 7918 and 7462 ions in ESI+ and ESI- mode, respectively, were successfully identified. Multivariate statistical analyses were applied, and 64 dysregulated metabolites, including fatty acids, amino acids, carboxylic acid, and phospholipids, amongst others, were identified. Pathway analysis further revealed that linoleic acid metabolism; propanoate metabolism; and glycine, serine, and threonine metabolism were acutely perturbed. The data obtained in this study offer insight into the defense mechanisms of A. cerana against V. destructor mites and provide a better method for understanding the synergistic effects of parasitism on honey bee colonies.
2017-10-27 | MTBLS379 | MetaboLights
Project description:Experiment was designed to study the effect of Deformed wing virus (DWV) and the mite Varroa destructor on global gene expression using microarray transcriptional profiling in developing worker honeybee (Apis mellifera). Newly hatched bee larvae (day 3 of bee development) were transferred from a Varroa-free colony with low DWV levels to a Varroa-infested colony with high levels of DWV in bees and Varroa mites. All transferred larvae were receiving the DWV strains present in this Varroa-infested colony with the food delivered by the nurse bees until their capping (day 8). About half of these larvae were capped with Varroa mite and were subjected to the mite piercing and feeding on their haemolymph during pupal development until sampling at purple eye stage (day 14). Exposure to the mite piercing and feeding resulted in about 1000-fold increase of the DWV levels in the majority of the mite-exposed pupae compared to the control pupae and the pupae not exposed to Varroa mites.
2013-06-01 | E-MTAB-1285 | biostudies-arrayexpress
Project description:Experiment was designed to study the effect of Deformed wing virus (DWV) and the mite Varroa destructor on on siRNA and miRNA composition using high-throughput sequencing of small RNA in developing worker honeybee (Apis mellifera). Newly hatched bee larvae (day 3 of bee development) were transferred from a Varroa-free colony with low DWV levels to a Varroa-infested colony with high levels of DWV in bees and Varroa mites. All transferred larvae were receiving the DWV strains present in this Varroa-infested colony with the food delivered by the nurse bees until their capping (day 8). About half of these larvae were capped with Varroa mite and were subjected to the mite piercing and feeding on their haemolymph during pupal development until sampling at purple eye stage (day 14). Exposure to the mite piercing and feeding resulted in about 1000-fold increase of the DWV levels in the majority of the mite-exposed pupae compared to the control pupae and the pupae not exposed to Varroa mites.
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Project description:The parasitic mite Varroa destructor causes both direct and indirect damage to infested honeybees but precise information about the identity of the metabolic pathways that are affected is still missing. In order to gain a deeper knwoledge about the effects of parasitization at various levels, while reducing to the miminum the impact of possible confounding factors, a standardized experiment was carried out under artificial condition.
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Project description:Using artificial Parafilm™ dummies, we explored the nutrition of V. destructor females and assessed their survival when fed on hemolymph from bee larvae, pupae or adults. We compared the results to fully synthetic solutions and to filtered larval plasma. The results showed that the parasites could survive for several days or weeks on our different diets. Hemolymph from larva yielded the highest survival rates and filtered cell-free hemolymph was sufficient to maintain the mites for 14 days or more. This cell free solution thus theoretically contains all necessary nutrients for the mite survival. A proteomic analysis on the larval plasma outlined a list of 54 proteins among which several energy metabolism proteins such as vitellogenin, hexamerin or transferrin. These molecules represent key candidate nutrients that could be crucial for V. destructor survival.
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Project description:Aims of the project is the identification of soluble chemosensory proteins in the olfactory organs of the honeybee mite Varroa destructor. Different families of soluble proteins acting as carriers for odorants have been identified in hexapods, while very limited information is available for other terrestrial Arthropoda. In mites and ticks olfactory organs are located on the distal part of the forelegs and on the capitulum appendages. These two body tagmata and the second pair of legs as control, have been dissected out of phoretics and reproductives Varroa destructor females and protein extracts have been analysed through shotgun proteomics. Protein identification and relative quantification (Label-free quantification, LFQ) has been performed using MaxQuant (version 1.5.8.3). Among the proteins more abundant in appendages bearing chemosensory organs, considering as a model hexapoda soluble olfactory proteins, we have searched for small secreted proteins whose structure include a hydrophobic binding pocket.
2018-04-12 | PXD008679 | Pride
Project description:Varroa destructor is one of the major parasitic pests in modern beekeeping worldwide. Since it shifted host from Asian to Western honey bees, it was shown to weaken colonies by feeding on both immature and adult stages while transmitting several deadly viruses in the process. Nutrition, an overlooked aspect of parasite biology, is thus a key to comprehend the V. destructor life cycle and its impacts on its honey bee host. We explored the feeding physiology of this ectoparasite both through artificial feeding and by dissecting their gut and analysing their protein content especially in terms of host-derived nutrients. We found that the mite requires higher quantities of carbohydrates than proteins and that the nature of these proteins is crucial for its survival. The abundance of these essential nutrients such as Vitellogenin or Hexamerin varies throughout the bee development and could directly impact the parasite physiology. The analysis and identification of key proteins required for the mite’s survival and reproduction will pave the way for the development of more specific control strategies.
2024-12-16 | PXD053798 | Pride
Project description:Genomic comparison of the sister species V. destructor and V. jacobsoni mite
| PRJDB6279 | ENA