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Identification of constitutively-expressed immune effectors in the house fly (Musca domestica) and the transcription factors that regulate them

ABSTRACT: Identification of constitutively-expressed immune effectors in the house fly (Musca domestica) and the transcription factors that regulate them

PROVIDER: PRJNA757009 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15567351_1.fastq.gz	Fastqsanger.gz
	SRR15567351_2.fastq.gz	Fastqsanger.gz
	SRR15567352_1.fastq.gz	Fastqsanger.gz
	SRR15567352_2.fastq.gz	Fastqsanger.gz
	SRR15567353_1.fastq.gz	Fastqsanger.gz

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Identification of constitutively-expressed immune effectors in the house fly (Musca domestica) and the transcription factors that regulate them

Project description:In this study we fed groups of female house flies one of two Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli) or control broth and used RNA-seq to determine differential gene expression at 4 hours post-feeding. Flies that ingested P. aeruginosa induced expression of genes encoding digestive proteins in the gut but did not induce AMPs. Flies in all treatment groups constitutively expressed some paralogs of lysozyme and AMP genes at the transcript level.

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Project description:Sex determination evolves rapidly, often because of turnover of the genes at the top of the pathway. The house fly, Musca domestica, has a multifactorial sex determination system, allowing us to identify the selective forces responsible for the evolutionary turnover of sex determination in action. There is a male determining factor, M, on the Y chromosome (Y^M), which is probably the ancestral state. An M factor on the third chromosome (III^M) has reached high frequencies in multiple populations across the world, but the evolutionary forces responsible for the invasion of III^M are not resolved. To test if the III^M chromosome invaded because of sex-specific selection pressures, we used mRNA sequencing to determine if isogenic males that differ only in the presence of the Y^M or III^M chromosome have different gene expression profiles. We find that more genes are differentially expressed between Y^M and III^M males in testis than head, and that genes with male-biased expression are most likely to be differentially expressed between Y^M and III^M males. This suggests that male phenotypes, especially those related to male fertility, are more likely to be affected by the male-determining chromosome, supporting the hypothesis that sex-specific selection acts on alleles linked to the male-determining locus driving evolutionary turnover in the sex determination pathway. We additionally find that III^M males have a "masculinization" gene expression profile, suggesting that the III^M chromosome has accumulated an excess of male-beneficial alleles because of its male-limited transmission.

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