Project description:We microdissected each embryo region from 6-micron paraffin sections using the Leica AS LMD system to identify all genes active in different embryo region of an SRB seed containing globular-stage embryos. Keywords: cell type comparison
Project description:We microdissected each embryo region from 6-micron paraffin sections using the Leica AS LMD system to identify all genes active in different embryo region of an SRB seed containing globular-stage embryos. Experiment Overall Design: Globular-stage embryo regions were isolated using the Leica AS LMD system. Total RNA was amplified and hybridized with Affymetrix Soybean Genome GeneChip Arrays.
Project description:Corneal epithelial stem cells reside in the limbus that is the transitional zone between the cornea and conjunctiva, and are essential to maintain the homeostasis of corneal epithelium. However, their characterization is poorly understood. Therefore, we constructed gene expression profiles of limbal epithelial SP and non-SP cell using RNA-sequencing. As a result, limbal epithelial SP cells have immature cell phenotypes with endothelial/mesenchymal cell markers, while limbal epithelial non-SP cells have epithelial progenitor cell markers.
