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Single cell RNAseq of Th17 and Th22 cells with Gpr65_WT versus KO

ABSTRACT: Single cell RNAseq of Th17 and Th22 cells with Gpr65_WT versus KO

PROVIDER: PRJNA757733 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15610200.fastq.gz	Fastqsanger.gz
	SRR15610201.fastq.gz	Fastqsanger.gz
	SRR15610202.fastq.gz	Fastqsanger.gz
	SRR15610203.fastq.gz	Fastqsanger.gz
	SRR15610204.fastq.gz	Fastqsanger.gz

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Single cell RNAseq of Th17 and Th22 cells with Gpr65_WT versus KO

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Th17, Th22 and Treg cells are enriched in the healthy human cecum

Project description:Dysregulation of CD4+ T cell populations leads to intestinal inflammation, but the regional differences of these T cell populations remained unclear in the healthy population. We first used flow cytometry to show that Th17 and Th22 cells are specifically enriched in the human cecum, while Th1 and Th2 cells do not differ regionally. We subsequently performed transcriptional profiling and found that Th17 was positively associated with the expression of RETN and negatively associated with TFF1, suggesting that the differential enrichment of Th17 cells in the healthy human cecum was underlined by the metabolic differences in this environment. Transcriptional profiling with microarray on human intestinal pinch biopsies obtained from different sites of the healthy colon.

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Th22 cells represent a distinct human T cell subset involved in epidermal immunity and remodeling

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