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Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [bulk RNA-seq]

ABSTRACT: Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [bulk RNA-seq]

PROVIDER: PRJNA757785 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15614100_1.fastq.gz	Fastqsanger.gz
	SRR15614100_2.fastq.gz	Fastqsanger.gz
	SRR15614101_1.fastq.gz	Fastqsanger.gz
	SRR15614101_2.fastq.gz	Fastqsanger.gz
	SRR15614102_1.fastq.gz	Fastqsanger.gz

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Derivation of functional trophoblast stem cells from human primed pluripotent stem cells

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Project description:Trophoblast stem cells (TSC) have recently been derived from human embryos, and early first trimester placenta; however, aside from ethical challenges, the unknown disease potential of these cells limits their scientific utility. We have previously established a BMP4-based two-step protocol for differentiation of primed human pluripotent stem cells (hPSC) into functional trophoblast; however, those trophoblast could not be maintained in a self-renewing TSC-like state. Here, we use the first step from this protocol, followed by a switch to a newly-developed TSC media, to derive bona-fide TSC. We show that these cells resemble placenta- and naïve hPSC-derived TSC, based on their transcriptome, as well as in vitro and in vivo differentiation potential. We conclude that primed hPSC can be used to generate functional TSC, through a simple protocol which can be applied to a widely-available set of existing hPSC, including induced pluripotent stem cells derived from patients with known birth outcomes.

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