Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation [scRNA-seq]

ABSTRACT: Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation [scRNA-seq]

PROVIDER: PRJNA759865 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation

Project description:Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation

| PRJNA759863 | ENA

Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation [RNA-seq]

Project description:Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation [RNA-seq]

| PRJNA759864 | ENA

Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation [DNaseI-seq]

Project description:Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation [DNaseI-seq]

| PRJNA759866 | ENA

Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation

Project description:Lineage commitment and differentiation is driven by the concerted action of master transcriptional regulators at their target chromatin sites. Multiple efforts have characterized the key transcription factors (TFs) that determine the various hematopoietic lineages. However, the temporal interactions between individual TFs and their chromatin targets during differentiation and how these interactions dictate lineage commitment remains poorly understood. We performed dense, daily, temporal profiling of chromatin accessibility (DNase I-seq) and gene expression changes (total RNA-seq) along ex vivo human erythropoiesis to comprehensively define developmentally regulated DNase I hypersensitive sites (DHSs) and transcripts. We link both distal DHSs to their target gene promoters and individual TFs to their target DHSs, revealing that the regulatory landscape is organized in distinct sequential regulatory modules that regulate lineage restriction and maturation. Finally, direct comparison of transcriptional dynamics (bulk and single-cell) and lineage potential between erythropoiesis and megakaryopoiesis uncovers differential fate commitment dynamics between the two lineages as they exit the stem and progenitor stage. Collectively, these data provide novel insights into the global regulatory landscape during hematopoiesis.

2021-09-30 | GSE182816 | GEO

Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation.

Project description:Lineage commitment and differentiation is driven by the concerted action of master transcriptional regulators at their target chromatin sites. Multiple efforts have characterized the key transcription factors (TFs) that determine the various hematopoietic lineages. However, the temporal interactions between individual TFs and their chromatin targets during differentiation and how these interactions dictate lineage commitment remains poorly understood. Here we perform dense, daily, temporal profiling of chromatin accessibility (DNase I-seq) and gene expression changes (total RNA-seq) along ex vivo human erythropoiesis to comprehensively define developmentally regulated DNase I hypersensitive sites (DHSs) and transcripts. We link both distal DHSs to their target gene promoters and individual TFs to their target DHSs, revealing that the regulatory landscape is organized in distinct sequential regulatory modules that regulate lineage restriction and maturation. Finally, direct comparison of transcriptional dynamics (bulk and single-cell) and lineage potential between erythropoiesis and megakaryopoiesis uncovers differential fate commitment dynamics between the two lineages as they exit the stem and progenitor stage. Collectively, these data provide insights into the temporally regulated synergy of the cis- and the trans-regulatory components underlying hematopoietic lineage commitment and differentiation.

| S-EPMC8611072 | biostudies-literature

Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation [DNaseI-seq]

2021-09-30 | GSE183268 | GEO

Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation [RNA-seq]

2021-09-30 | GSE183266 | GEO

Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation [scRNA-seq]

2021-09-30 | GSE183267 | GEO

Discrete tissue microenvironments instruct diversity in resident memory T cell function and plasticity

Project description:Discrete tissue microenvironments instruct diversity in resident memory T cell function and plasticity

| PRJNA740308 | ENA

Discrete tissue microenvironments instruct diversity in resident memory T cell function and plasticity (Liver)

Project description:Discrete tissue microenvironments instruct diversity in resident memory T cell function and plasticity (Liver)