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Genome-wide expression analysis of Arabidopsis Col-0 plant and clf mutant plant under high humidity [RNA-seq]

ABSTRACT: Genome-wide expression analysis of Arabidopsis Col-0 plant and clf mutant plant under high humidity [RNA-seq]

PROVIDER: PRJNA761292 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15736802_1.fastq.gz	Fastqsanger.gz
	SRR15736802_2.fastq.gz	Fastqsanger.gz
	SRR15736803_1.fastq.gz	Fastqsanger.gz
	SRR15736803_2.fastq.gz	Fastqsanger.gz
	SRR15736804_1.fastq.gz	Fastqsanger.gz

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Genome-wide expression analysis of Arabidopsis Col-0 plant and clf mutant plant under high humidity [RNA-seq]

Project description:Purpose: The goal of this study is to demonstrate the global expression profile of Arabidopsis wild type and clf mutant plants in response to high air humidity Methods: Four-week-old Arabidopsis plant leaves were treated with ~95% relative humidity for 0.5 hour (0h as control) Results and Conclusions: This study showed that many genes including ABA-responsive genes and stomotal movement-related genes were differentially expressed in the clf mutant plant.

2024-09-06 | GSE183558 | GEO

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Project description:We report the H3K27 trimethylation profiles in Arabidospsis wild type and clf mutant plants before and after high humidity treatment. We find that many genes display a different H3-lysine 27 trimethylation level in the clf mutant plant compared to Col-0 plant. In addition, high humidity also influences H3K27 trimethylation profiles in both genotypes.

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Genome-wide analysis reveals CLF-regulated transcripts in Arabidopsis

Project description:CURLY LEAF (CLF), the major histone methyltransferase of Polycomb Repressive Complex 2 (PRC2), modifies trimethylation of histone H3 lysine 27 (H3K27me3) and mediates dynamical chromatin repression in Arabidopsis. Here we profiled Arabidopsis transcriptomes obtained from roots, leaves, flowers and siliques of Col-0 and clf-28 plants using RNA-seq. Our analysis uncovered 3835 transcription units were up-regulated in clf-28. Compared with H3K27me3 ChIP-CHIP data, we found at least 42% of them were associated with H3K27me3.

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Project description:CURLY LEAF (CLF), the major histone methyltransferase of Polycomb Repressive Complex 2 (PRC2), modifies trimethylation of histone H3 lysine 27 (H3K27me3) and mediates dynamical chromatin repression in Arabidopsis. Here we used strand specific RNA-sequencing to profile Arabidopsis transcriptomes obtained from roots, shoots, flowers and siliques of Col-0 and clf-28 plants. Our analysis identified a large number of CLF-regulatedd transcripts in Arabidopsis.

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Bisulfite (methylome) sequencing of progenies of Col-0 grafted on msh1; Col-0 grafted on dcl2,3,4,msh1; Col-0 grafted on Col-0; msh1 mutant; dcl2,3,4,msh1 quadruple mutant in Arabidopsis [msh1_graft_BS-seq]

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