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MRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription.

ABSTRACT: mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription.

PROVIDER: PRJNA761329 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15737000.fastq.gz	Fastqsanger.gz
	SRR15737001.fastq.gz	Fastqsanger.gz
	SRR15737002.fastq.gz	Fastqsanger.gz
	SRR15736971.fastq.gz	Fastqsanger.gz
	SRR15736972.fastq.gz	Fastqsanger.gz

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mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription.

Project description:Several transcription inhibitors have been developed as cancer therapies. However, they show modest clinical activity highlighting that our understanding of the cellular response to transcriptional inhibition remains incomplete. Here we report that potent inhibitors of transcription not only impact mRNA output, but also markedly impair mRNA transcript localisation and nuclear export. We demonstrate that retention of newly transcribed mRNA in nuclear speckles is an adaptive response to chemically distinct transcriptional inhibitors. Retained transcripts are fully processed and accumulate in proportion to the expression level of the genes from which they emanate. The TREX mRNA export complex plays an integral role in directing nascent transcripts to nuclear speckles where they are bound to NXF1, protected from degradation, and poised for rapid export following re-initiation of transcription. Our findings provide new insights into the crosstalk between transcription and mRNA export, with important implications for drugs aiming to inhibit transcription for therapeutic gain.

2025-02-13 | GSE183570 | GEO

mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription.

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mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription [SLAM-seq]

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mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription.

Project description:mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription.

| PRJNA761339 | ENA

mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription.

Project description:mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription.

| PRJNA761338 | ENA

mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription [SLAM-seq]

Project description:mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription [SLAM-seq]

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mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription.

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-02-12 | GSE183571 | GEO

Nuclear Speckles enable processing of RNA from GC-rich Isochores [SON_Halo_4sU_pulse]

Project description:Nuclear Speckles are large, multi-component condensates in the mammalian nuclei that are thought to be involved in various stages of gene expression, including transcription, splicing, and export. Investigating the direct role of Speckles in these processes have been intractable due to their heterogeneous, multi-component nature. Here we show that Speckles are necessary for the splicing of GC-rich, levelled intron/exon architecture. Acute removal of Speckles leads to a drastic downregulation of GC-rich genes which are clustered within GC-rich isochores. Genes outside these domains are physically away from Speckles and are not affected from loss of Speckles. Animal genomes that do not have GC-rich isochores, do not have GC-rich levelled intron/exon architecture and do not have Speckles. Our results suggest that the expansion of Speckles core proteins’ intrinsically disordered domains during vertebrate evolution facilitated GC-content elevation in Amniotes by creating a condensate necessary to splice the by-products of this process: GC-rich, levelled intron/exon architecture.

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