Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Deletion and Complementation of Alr3614 in Anabaena PCC7120

ABSTRACT: Deletion and Complementation of Alr3614 in Anabaena PCC7120

PROVIDER: PRJNA761425 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Deletion and Complementation of Alr3614 in Anabaena PCC7120

Project description:To examine Alr3614 influence on the expression of Anabaena sp. PCC 7120, we have used customized microarrays to identify genes potentially regulated by this short DNA-binding protein. We compared the expression of an Alr3614-deletion strain with an Alr3614-complementation strain.

2022-05-12 | GSE183629 | GEO

Unbalancing FurB (Zur)-mediated homeostasis in Anabaena sp. PCC7120: Consequences on heterocyst development and biofilm formation

Project description:Here, we report the comparison of transcriptomes of Anabaena sp. PCC7120 and the FurB(Zur) deletion derivative strain (MN38). Anabaena sp PCC7120 is a cyanobacterium that differentiates specialized nitrogen-fixing cells called heterocysts and that is capable of forming biofilms. Our data showed that the deletion of FurB negativily affected the heterocyst development and the biofilm formation. In addition, the RNA-seq data together with gel retardation assays unveiled that FurB is directly involved in the regulation of several genes related to heterocyst development and biofilm formation and other novel functions different from the ones related to the canonical Zur regulon.

2023-09-14 | E-MTAB-12347 | biostudies-arrayexpress

Anabaena PCC7120 infection with Cyanophage A1

Project description:Anabaena PCC7120 infection with Cyanophage A1

| PRJNA761428 | ENA

Comparative transcriptomics of Anabaena sp. PCC7120 grown as biofilm under bubbling or their concurrent planktonic fractions

Project description:Bacterial biofilms are microbial communities that grow attached to a surface, embedded in a complex matrix composed of extracellular polymeric compounds such as exopolysaccharides. They are commonly induced in response to stresses such as desiccation or depredation, and display increased resilience. Model cyanobacterium Anabaena sp. PCC7120, typically cultured planktonically, is able to form biofilms. In order to advance in the understanding of phototrophic biofilm formation, we carried out comparative transcriptomic analysis of biofilm and planktonic Anabaena and observed vast alterations. A total of 1099 differentially expressed genes (DEGs) were identified, of which 641 could be assigned a functional annotation. Major central metabolic pathways were affected, including carbohydrate metabolism, photosynthesis, ribosome biogenesis andtranslation. nitrogen metabolism and cellular envelops maintenance, among others.

2026-05-08 | E-MTAB-15589 | biostudies-arrayexpress

Anabaena PCC7120 infection with Cyanophage A1

Project description:In order to examine the response of Anabaena sp. PCC 7120 to an infection of the Cyanophage A1, we have used customized microarrays to identify genes potentially up- or downregulated by the infection. For this we compared cell culture before and after infection.

2023-11-01 | GSE183630 | GEO

RNA-seq of Nostoc sp. PCC 7120 (Anabaena sp. PCC 7120) wild-type strain compared to a FurC-overexpressing derivative strain grown in BG11 and after 48 hours of nitrogen-step down.

Project description:Here, we report the comparison of transcriptomes of Anabaena sp. PCC7120 and a FurC-overexpressing derivative strain grown under standard conditions (BG11) and after 48 hours of nitrogen step-down (BG110). Anabaena sp PCC7120 is a cyanobacterium that differentiates specialized nitrogen-fixing cells called heterocysts. Our data suggests that FurC directly controls the regulation of heterocyst differentiation and nitrogen fixation in this cyanobacterium. In addition, we found that FurC is also clearly involved in the regulation of several genes belonging to different functional categories, such as iron metabolism, photosynthesis and regulatory functions.

2020-04-28 | E-MTAB-8996 | biostudies-arrayexpress

The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120

Project description:Deletion of the global transcription factor PacR in Anabaena triggers heterocyst formation even in NO3- containing medium, likely due to impaired NO3- uptake and disrupted NH4+ assimilation in the GOGAT cycle. This phenotype may be exacerbated by reduced PSI-yield and reduced expression of ferredoxin, which may lead to less reducing equivalents for nitrogen uptake and fixation. These results highlight PacR’s role as a global regulator of carbon metabolism and photosynthesis while also establishing its involvement in regulating nitrogen metabolism.

2024-11-05 | GSE280386 | GEO

Comparative transcriptomics of Anabaena sp. PCC7120 grown as biofilm under bubbling or their concurrent planktonic fractions

Project description:Comparative transcriptomics of Anabaena sp. PCC7120 grown as biofilm under bubbling or their concurrent planktonic fractions

| PRJEB97479 | ENA

Unbalancing FurB (Zur)-mediated homeostasis in Anabaena sp. PCC7120: Consequences on heterocyst development and biofilm formation

Project description:Unbalancing FurB (Zur)-mediated homeostasis in Anabaena sp. PCC7120: Consequences on heterocyst development and biofilm formation

| PRJEB57199 | ENA

The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120

Project description:The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120

| PRJNA1178285 | ENA