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Mesenchymal-epithelial crosstalk shapes intestinal regionalisation via Wnt and Shh signalling

ABSTRACT: Mesenchymal-epithelial crosstalk shapes intestinal regionalisation via Wnt and Shh signalling

PROVIDER: PRJNA761659 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13386926.fastq.gz	Fastqsanger.gz
	SRR13386927.fastq.gz	Fastqsanger.gz
	SRR15731520.fastq.gz	Fastqsanger.gz
	SRR15731521.fastq.gz	Fastqsanger.gz
	SRR15731522.fastq.gz	Fastqsanger.gz

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Project description:Mesothelioma is an aggressive cancer of the mesothelial layer associated with an extensive fibrotic response. The latter is in large part mediated by cancer-associated fibroblasts which mediate tumour progression and poor prognosis. However, understanding of the crosstalk between cancer cells and fibroblasts in this disease is mostly lacking. Here, using co-cultures of patient-derived mesothelioma cell lines and lung fibroblasts, we demonstrate that fibroblast activation is a self-propagated process producing a fibrotic extracellular matrix (ECM) and triggering drug resistance in mesothelioma cells. Following characterisation of mesothelioma cells/fibroblasts signalling crosstalk, we identify several FDA-approved targeted therapies as far more potent than standard-of-care Cisplatin/Pemetrexed in ECM-embedded co-culture spheroid models. In particular, the SRC family kinase inhibitor, Saracatinib, extends overall survival well beyond standard-of-care in a mesothelioma genetically-engineered mouse model. In short, we lay the foundation for the rational design of novel therapeutic strategies targeting mesothelioma/fibroblast communication for the treatment of mesothelioma patients.

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