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Transcriptome analysis of polymorphic p53 codon 72 under genotoxic stress

ABSTRACT: Transcriptome analysis of polymorphic p53 codon 72 under genotoxic stress

PROVIDER: PRJNA761662 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15809382_1.fastq.gz	Fastqsanger.gz
	SRR15809382_2.fastq.gz	Fastqsanger.gz
	SRR15809383_1.fastq.gz	Fastqsanger.gz
	SRR15809383_2.fastq.gz	Fastqsanger.gz
	SRR15809384_1.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of polymorphic p53 codon 72 under genotoxic stress

Project description:We report the transcriptome data produced from isogenic polymorphic p53 codon 72 iPSCs under doxorubicin treatment. By inserting BAC DNA into one allele of the heterozygous polymorphic p53 codon 72, each clone expressed a p53 protein encoding P72 or R72 from another allele in which it was not inserted. The transcriptional regulation of p53 target genes was compared between the isogenic lines under doxorubicin treatment.

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The codon 72 polymorphism of p53 regulates interactions with NF-κB and transactivation of genes involoved in immunity and inflamation

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Project description:A common polymorphism at codon 72 in the p53 tumor suppressor gene encodes either proline(P72) or arginine(R72). These two variants might possess altered biological function which influences p53’s transcriptional, senescence and apoptotic functions. We found that P72 has increased apoptosis when compare to R72. To further identify unknown p53 target genes which might play a role in the increased apoptosis in P72 thymocytes, we have employed whole genome microarray expression profiling as a discovery platform. Mouse thymocytes were isolated from the thymuses of mice that were untreated (0 hour) or following 2 or 4 hours of exposure to 5 Gray of gamma radiation. Mouse thymocytes were isolated from the thymuses of mice that were untreated (0 hour) or following 2 or 4 hours of exposure to 5 Gray of gamma radiation. Four independent experiments were performed at each time point (P72 and R72) and at each timepoint 2 mice of each genotype were used, and their thymocytes were pooled together.

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