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A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [Bulk RNA-Seq II]

ABSTRACT: A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [Bulk RNA-Seq II]

PROVIDER: PRJNA761760 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15824027.fastq.gz	Fastqsanger.gz
	SRR15824028.fastq.gz	Fastqsanger.gz
	SRR15824029.fastq.gz	Fastqsanger.gz
	SRR15824030.fastq.gz	Fastqsanger.gz
	SRR15824031.fastq.gz	Fastqsanger.gz

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A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella

Project description:A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella

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A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [scRNA-Seq]

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A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [scRNA-Seq]

Project description:Interactions between intracellular bacteria and mononuclear phagocytes give rise to diverse cellular phenotypes that may determine the outcome of infection. Recent advances in single-cell RNA-seq (scRNA-seq) have identified multiple subsets within the mononuclear population, but implications to their function during infection remain unknown. Here, we applied microscopy, flow cytometry and scRNA-seq to survey the mononuclear niche of intracellular Salmonella Typhimurium (S.Tm) during early systemic infection in mice. We describe eclipse-like growth kinetics in the spleen, with a first phase of bacterial control mediated by tissue-resident red-pulp macrophages. A second phase involved extensive bacterial replication within a newly identified macrophage population characterized by CD9 expression. Using Nr4a1e2-/- mice we established that CD9+ macrophages originated from non-classical monocytes (NCM), and NCM-depleted mice were more resistant to S.Tm infection. Our study defines a novel host-pathogen interface, with macrophage subset-specific interactions that determines early infection dynamics and the infection outcome of the entire organism.

2021-11-16 | GSE164254 | GEO

A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [Bulk RNA-Seq II]

2021-11-16 | GSE183728 | GEO

A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [Bulk RNA-Seq I]

2021-11-04 | GSE164253 | GEO

A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [Bulk RNA-Seq I]

Project description:A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [Bulk RNA-Seq I]

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A non-classical monocyte-derived macrophage subset provides a splenic replication niche for intracellular Salmonella.

Project description: Not available

| S-EPMC8691386 | biostudies-literature

A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-11-04 | GSE164255 | GEO

RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice

Project description:The transcriptome of splenic classical dendritic cells (cDCs) (CD3- CD19- CD45+ MHC-II+ CD11c+) was compared in Wild Type (WT) mice, Germ Free (GF) mice and mice deficient for the Ifnar1 gene (IFNAR). Cells were isolated and sorted by flow cytometry to high purity. The cells were stimulated ex vivo with PBS or LPS. The results show remarkable similarities between the transcriptome of splenic cDCs in GF and IFNAR mice.

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Gene expression profiling of the classical (CD14++CD16-), intermediate (CD14++CD16+) and nonclassical (CD14+CD16+) human monocyte subsets

Project description:The new official nomenclature subdivides human monocytes into three subsets, classical (CD14++CD16-), intermediate (CD14++CD16+) and nonclassical (CD14+CD16+). Here, we comprehensively define relationships and unique characteristics of the three human monocyte subsets using microarray and flow cytometry analysis. Our analysis revealed that the intermediate and nonclassical monocyte subsets were most closely related. For the intermediate subset, majority of genes and surface markers were expressed at an intermediary level between the classical and nonclassical subset. There features therefore indicate a close and direct lineage relationship between the intermediate and nonclassical subset. From gene expression profiles, we define unique characteristics for each monocyte subset. Classical monocytes were functionally versatile, due to the expression of a wide range of sensing receptors and several members of the AP-1 transcription factor family. The intermediate subset was distinguished by high expression of MHC class II associated genes. The nonclassical subset were most highly differentiated and defined by genes involved in cytoskeleton rearrangement that explains their highly motile patrolling behavior in vivo. Additionally, we identify unique surface markers, CLEC4D, IL-13RA1 for classical, GFRA2, CLEC10A for intermediate and GPR44 for nonclassical. Our study hence defines the fundamental features of monocyte subsets necessary for future research on monocyte heterogeneity.

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