Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity [scRNA-seq]

ABSTRACT: Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity [scRNA-seq]

PROVIDER: PRJNA762258 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15858091_1.fastq.gz	Fastqsanger.gz
	SRR15858091_2.fastq.gz	Fastqsanger.gz
	SRR15858092_1.fastq.gz	Fastqsanger.gz
	SRR15858092_2.fastq.gz	Fastqsanger.gz
	SRR15858093_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity

Project description:Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity

| PRJNA762252 | ENA

Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity [RNA-Seq]

Project description:Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity [RNA-Seq]

| PRJNA762257 | ENA

Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity [ATAC-seq]

Project description:Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity [ATAC-seq]

| PRJNA762259 | ENA

Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity [Mint-ChIP)

Project description:Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity [Mint-ChIP)

| PRJNA791347 | ENA

Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity [scRNA-seq]

Project description:We perfromed scRNA-seq on normal and Kras mutant mouse lung epithelial cells.

2021-12-22 | GSE183875 | GEO

Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity [RNA-Seq]

Project description:We perfromed RNA-seq on normal and Kras mutant mouse lung epithelial cells.

2021-12-22 | GSE183874 | GEO

Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity [ATAC-seq]

Project description:We perfromed ATAC-seq on normal and Kras mutant mouse lung epithelial cells.

2021-12-22 | GSE183873 | GEO

Epigenetic basis of oncogenic-Kras mediated epithelial-cellular proliferation and plasticity [Mint-ChIP)

Project description:We perfromed Mint-ChIP using anti-FOSL1 antibody on Kras mutant mouse lung epithelial cells.

2021-12-22 | GSE192406 | GEO

Epigenetic basis of oncogenic-Kras-mediated epithelial-cellular proliferation and plasticity.

Project description:Oncogenic Kras induces a hyper-proliferative state that permits cells to progress to neoplasms in diverse epithelial tissues. Depending on the cell of origin, this also involves lineage transformation. Although a multitude of downstream factors have been implicated in these processes, the precise chronology of molecular events controlling them remains elusive. Using mouse models, primary human tissues, and cell lines, we show that, in Kras-mutant alveolar type II cells (AEC2), FOSL1-based AP-1 factor guides the mSWI/SNF complex to increase chromatin accessibility at genomic loci controlling the expression of genes necessary for neoplastic transformation. We identified two orthogonal processes in Kras-mutant distal airway club cells. The first promoted their transdifferentiation into an AEC2-like state through NKX2.1, and the second controlled oncogenic transformation through the AP-1 complex. Our results suggest that neoplasms retain an epigenetic memory of their cell of origin through cell-type-specific transcription factors. Our analysis showed that a cross-tissue-conserved AP-1-dependent chromatin remodeling program regulates carcinogenesis.

| S-EPMC8938988 | biostudies-literature

Transcription profiling of human lung epithelial cells ollowing oncogenic KRAS expression and TBK1 suppression or infection with oncogenic KRAS (G12V) - SuperSeries

Project description:The proto-oncogene KRAS is mutated in a wide array of human cancers, most of which are aggressive and respond poorly to standard therapies. Although the identification of specific oncogenes has led to the development of clinically effective, molecularly targeted therapies in some cases, KRAS has remained refractory to this approach. An alternative strategy for targeting KRAS is to identify gene products that, when suppressed or inhibited, result in cell death only in the presence of an oncogenic allele. Here we have used systematic RNA interference (RNAi) to detect synthetic lethal partners of oncogenic KRAS and found that the non-canonical IkB kinase, TBK1, was selectively essential in cells that harbor mutant KRAS. Suppression of TBK1 induced apoptosis specifically in human cancer cell lines that depend on oncogenic KRAS expression. In these cells, TBK1 activated NF- B anti-apoptotic signals involving cREL and BCL-XL that were essential for survival, providing mechanistic insights into this synthetic lethal interaction. These observations identify TBK1 as a potential therapeutic target in KRAS mutant tumors and establish a general approach for the rational identification of co-dependent pathways in cancer. This SuperSeries is composed of the following subset Series:; GSE17643: Profiling of immortalized human lung epithelial cells following oncogenic KRAS expression and TBK1 suppression; GSE17671: Profiling of immortalized human lung epithelial cells following infection with oncogenic KRAS (G12V) Experiment Overall Design: Refer to individual Series

2009-10-31 | E-GEOD-17672 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data