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Systems-level Responses to Salinity and Temperature Variations in the Marine Bacterium Vibrio natriegens

ABSTRACT: Systems-level Responses to Salinity and Temperature Variations in the Marine Bacterium Vibrio natriegens

PROVIDER: PRJNA763369 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15902426_1.fastq.gz	Fastqsanger.gz
	SRR15902426_2.fastq.gz	Fastqsanger.gz
	SRR15902427_1.fastq.gz	Fastqsanger.gz
	SRR15902427_2.fastq.gz	Fastqsanger.gz
	SRR15902428_1.fastq.gz	Fastqsanger.gz

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Project description:This study employs integrated multi-omics analysis to systematically investigate the proteome and metabolome of marine bacterium Vibrio natriegens strain VCOD-2 (ATCC 14048) under optimal salinity (3% NaCl) and high-salinity stress (8% NaCl) conditions, aiming to reveal the molecular mechanisms of its high-salinity adaptation.Proteomics: Two salinity treatment groups were established with three biological replicates each, totaling six samples. Through trypsin digestion, TMTpro 16plex isobaric labeling, and high-pH reverse-phase fractionation, LC-MS/MS analysis was performed using Q Exactive HF-X Orbitrap mass spectrometer, successfully identifying 3077 protein groups and 31,646 peptides. Using 3% NaCl as control and 8% NaCl as experimental group, differentially expressed proteins (DEPs) were screened with fold change ≥1.5 or ≤0.667 and q-value<0.05, identifying 979 DEPs including 370 up-regulated and 609 down-regulated proteins. Differential proteins were further analyzed by COG, GO, and KEGG functional annotation and pathway enrichment analysis.Metabolomics: Intracellular metabolite profiles under 3% and 8% NaCl salinities were simultaneously determined. Through liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, multiple metabolites related to salt stress response were identified and quantified, including compatible solutes, amino acids, organic acids, sugars, and other metabolites. Metabolomics analysis revealed metabolic pathway reprogramming under salt stress.Multi-omics Integration: Through integrated analysis of proteomics and metabolomics data, the molecular response network of V. natriegens under high-salinity stress was systematically elucidated. The combined analysis of proteomics and metabolomics revealed changes in key metabolic pathways including osmotic regulation, ion transport, energy metabolism, compatible solute biosynthesis and accumulation, providing multi-layer molecular evidence for deeply understanding the high-salinity adaptation strategies of V. natriegens.This study provides important multi-omics resources for understanding salt adaptation mechanisms in V. natriegens and offers theoretical references for salt tolerance research and industrial applications of extremophilic microorganisms.

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Systems-level Responses to Salinity and Temperature Variations in the Marine Bacterium Vibrio natriegens

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