Ontology highlight
ABSTRACT:
PROVIDER: PRJNA763985 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR16219740_1.fastq.gz | Fastqsanger.gz | |||
| SRR16219740_2.fastq.gz | Fastqsanger.gz | |||
| SRR16219741_1.fastq.gz | Fastqsanger.gz | |||
| SRR16219741_2.fastq.gz | Fastqsanger.gz | |||
| SRR16219742_1.fastq.gz | Fastqsanger.gz |
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Project description:The invasive Halyomorpha halys Stål, the Brown Marmorated Stink Bug (BMSB), and the native Nezara viridula L., the Southern Green Stink Bugs (SGSB), are widely distributed in Europe, even if the date of introduction and their diet differ. Saliva of Hemipteran pests plays an essential role in the interaction between insects and their host plants. Several aphid salivary proteomes have been identified and found to differ according to the species, while no comparative investigation between phytophagous stink bugs has been performed yet. Here we analyzed the salivary proteins from two bugs, BMSB and SGSB, using LC-MS/MS. A total of 238 and 305 proteins were identified from dissected salivary glands from BMSB and SGSB respectively. Among these, a large majority was found in both species. In comparison with salivary proteome from other Hemiptera, the most striking feature of the salivary gland proteomes from SGSB and BMSB is the similar protein functions patterns. Some of the proteins are speculated to be dependent of the feeding strategies, playing a significant role in plant-insect interactions. Our results provide a framework for future research to elucidate the molecular basis of differential impact of piercing-sucking insects on host plants.
2019-02-25 | PXD011976 | Pride
Project description:The invasive Halyomorpha halys Stål, the Brown Marmorated Stink Bug (BMSB), and the native Nezara viridula L., the Southern Green Stink Bugs (SGSB), are widely distributed in Europe, even if the date of introduction and their diet differ. Saliva of Hemipteran pests plays an essential role in the interaction between insects and their host plants. Several aphid salivary proteomes have been identified and found to differ according to the species, while no comparative investigation between phytophagous stink bugs has been performed yet. Here we analyzed the salivary proteins from two bugs, BMSB and SGSB, using LC-MS/MS. A total of 238 and 305 proteins were identified from dissected salivary glands from BMSB and SGSB respectively. Among these, a large majority was found in both species. In comparison with salivary proteome from other Hemiptera, the most striking feature of the salivary gland proteomes from SGSB and BMSB is the similar protein functions patterns. Some of the proteins are speculated to be dependent of the feeding strategies, playing a significant role in plant-insect interactions. Our results provide a framework for future research to elucidate the molecular basis of differential impact of piercing-sucking insects on host plants.
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Project description:A frightening resurgence of bed bug infestations has occurred over the last 10 years in the US. Current chemical methods have been inadequate for controlling bed bugs due to widespread insecticide resistance. Little is known about the mechanisms of resistance present in US bed bug populations, making it extremely difficult to develop intelligent strategies to control this pest. We have identified bed bugs collected in Richmond, VA which exhibit both kdr-type (L925I) and metabolic resistance to pyrethroid insecticides. LD50 bioassays determined resistance ratios of ~6000-fold to the insecticide deltamethrin, with contact bioassays confirming cross-resistance to several other labeled formulations. To identify metabolic genes potentially involved in the detoxification of pyrethroids, we performed deep-sequencing of the adult bed bug transcriptome, obtaining more than 2.5 million reads on the 454 titanium platform. Following assembly, analysis of newly identified gene transcripts in both Harlan (susceptible) and Richmond (resistant) bed bugs revealed several candidate cytochrome P450 and carboxyesterase genes which were significantly over-expressed in the resistant strain, consistent with the idea of increased metabolic resistance. These data will accelerate efforts to understand the biochemical basis for insecticide resistance in bed bugs, and provide molecular markers to assist in the surveillance of metabolic resistance.
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