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Study of TUT tailing on miRNA (Rescue TUT activity)

ABSTRACT: Study of TUT tailing on miRNA (Rescue TUT activity)

PROVIDER: PRJNA765021 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15998331.fastq.gz	Fastqsanger.gz
	SRR15998332.fastq.gz	Fastqsanger.gz
	SRR15998333.fastq.gz	Fastqsanger.gz
	SRR15998334.fastq.gz	Fastqsanger.gz
	SRR15998335.fastq.gz	Fastqsanger.gz

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Study of TUT tailing on miRNA (Rescue TUT activity)

Project description:Many microRNAs (miRNAs) exist alongside abundant miRNA isoforms (isomiRs), most of which arise from post-maturation sequence modifications, such as 3’ uridylation and adenylation. However, the ways in which these sequence modifications affect miRNA function remain poorly understood. To this aim, we have generated knock-out cell lines of TUT4, TUT7 and TENT2 (TUT2), and rescued with plasmids expressing the cDNA of such enzymes. Here, using these different cell lines, we have discovered that some of the redundant functions and specific functions of each tailing enzyme. Our study provides a comprehensive characterization of tailing on miRNAs.

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Project description:Many microRNAs (miRNAs) exist alongside abundant miRNA isoforms (isomiRs), most of which arise from post-maturation sequence modifications, such as 3’ uridylation and adenylation. However, the ways in which these sequence modifications affect miRNA function remain poorly understood. To this aim, we have generated single knock-out cell lines of TUT4, TUT7 and TENT2 (TUT2), as well as double knock-out (DKO) and triple knock-out (TKO) cell lines. Here, using these different cell lines, we have discovered that some of the redundant functions and specific functions of each tailing enzyme. Our study provides a comprehensive characterization of tailing on miRNAs.

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