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Dual role of Ovol2 on the germ cell lineage segregation during gastrulation in mouse embryogenesis.

ABSTRACT: Dual role of Ovol2 on the germ cell lineage segregation during gastrulation in mouse embryogenesis.

PROVIDER: PRJNA765589 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16019700.fastq.gz	Fastqsanger.gz
	SRR16019701.fastq.gz	Fastqsanger.gz
	SRR16019702.fastq.gz	Fastqsanger.gz
	SRR16019703.fastq.gz	Fastqsanger.gz
	SRR16019704.fastq.gz	Fastqsanger.gz

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Dual role of Ovol2 on the germ cell lineage segregation during gastrulation in mouse embryogenesis. [RNA-Seq]

Project description:In mammals, primordial germ cells (PGCs), the origin of the germ line, are specified from the epiblast at the posterior region where gastrulation simultaneously occurs, yet the functional relationship between PGC specification and gastrulation remains unclear. Here, we show that Ovol2, a transcription factor conserved across the animal kingdom, balances these major developmental processes by repressing the epithelial-to-mesenchymal transition (EMT) driving gastrulation and the upregulation of genes associated with PGC specification. Ovol2a, a splice variant encoding a repressor domain, directly regulates EMT-related genes and consequently induces re-acquisition of potential pluripotency during PGC specification, whereas Ovol2b, another splice variant missing the repressor domain, directly upregulates genes associated with PGC specification. Taken together, these results elucidate the molecular mechanism underlying allocation of the germ line among epiblast cells differentiating into somatic cells through gastrulation.

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Dual role of Ovol2 on the germ cell lineage segregation during gastrulation in mouse embryogenesis. [ChIP-Seq]

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Dual role of Ovol2 on the germ cell lineage segregation during gastrulation in mouse embryogenesis. [RNA-Seq]

Project description:Dual role of Ovol2 on the germ cell lineage segregation during gastrulation in mouse embryogenesis. [RNA-Seq]

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Dual role of Ovol2 on the germ cell lineage segregation during gastrulation in mouse embryogenesis. [ChIP-Seq]

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Dual role of Ovol2 on the germ cell lineage segregation during gastrulation in mouse embryogenesis.

Project description:This SuperSeries is composed of the SubSeries listed below.

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Ex utero Mouse Embryogenesis from Pre-Gastrulation to Late-Organogenesis

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Project description:The development of stem-cell-derived models of mammalian embryogenesis has provided invaluable tools for investigating embryo development. However, constructing embryo models that can continuously recapitulate the full developmental trajectory, from zygotic genome activation (ZGA) to gastrulation, remains a major challenge. Here, we developed a new chemical cocktail to induce totipotent-like cells with robust proliferation ability, and leveraged these cells to establish a stepwise protocol to generate a continuous embryo model. This model sequentially mimics mouse embryogenesis from embryonic day 1.5 to 7.5. It recapitulates key developmental milestones, including ZGA in 2-cell embryos, the diversification of embryonic and extraembryonic lineages from 4-cell to 64-cell stages, the formation of blastocysts, and the subsequent development into post-implantation egg cylinders. Notably, these structures can undergo gastrulation, as evidenced by primitive streak formation and the subsequent emergence of several hallmarks of early organogenesis. Our study opens new avenues for modeling mammalian embryogenesis in vitro.

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