Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Co-culture of iAT2 with fibroblast isolated from healthy and COPD lung in alveosphere culture system

ABSTRACT: Co-culture of iAT2 with fibroblast isolated from healthy and COPD lung in alveosphere culture system

PROVIDER: PRJNA767829 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Co-culture of iAT2 with fibroblast isolated from healthy and COPD lung in alveosphere culture system

Project description:transciptomic approach applied to our sophisticated in vitro model of iPSC-alveospheres with fibroblasts, we explored epithelial-mesenchymal cross-talk in alveolar niche in COPD.

2024-09-30 | GSE185151 | GEO

CRISPRi DSP-kd in iAT2

Project description:CRISPRi DSP-kd in iAT2

| PRJNA781443 | ENA

Isolated Blood Immune Cell Transcriptomics in COPD

Project description:We observed separation of COPD patients from control smokers by CD4+ T cell transcriptomics. In contrast to CD4+ T cells, there were no transcript differences in whole blood, CD8+ T cells or CD14+ monocytes.

2016-04-15 | GSE56767 | GEO

Transcriptome analysis of primary bronchial epithelial cells from healthy, ex-smoker COPD and current smoker COPD patients cultured in ALI conditions

Project description:Transcriptome analysis of primary bronchial epithelial cells from healthy, ex-smoker COPD and current smoker COPD patients cultured in ALI conditions

2026-04-29 | GSE301910 | GEO

Comparison of Treg and Teff cells in healthy and COPD smokers.

Project description:Rationale: COPD is characterized by an abnormal regulatory T cell (Treg) response and increases in Th1 and Th17 cell responses. It is unclear if dysregulation of miRNAs within Treg alters the adaptive immunophenotype in COPD. Objectives: To compare the miRNA profile of COPD Treg cells with that of healthy controls and to explore the function of differentially expressed miRNAs. Methods: Treg cells (CD4+CD25+CD49-CD127-) and T effector (CD4+CD25-) cells were obtained from the peripheral blood of 4 nonsmokers, 4 healthy current smokers, and 4 COPD current smokers for analysis of miRNA expression, matching them for age and sex. We assessed expression initially by microarray analysis on the Illumina miRNA platform then conducted real time RT-PCR validation of the microarray results.

2014-04-19 | GSE56923 | GEO

Comparison of Treg and Teff cells in healthy and COPD smokers.

2014-04-19 | E-GEOD-56923 | biostudies-arrayexpress

CRISPRi DSP-kd in iAT2

Project description:We performed single cell transcriptomic profiling of induced human pluripotent stem cells (iPSCs)-derived type 2 alveolar epithelial cells (iAT2). iPSCs stably expressed CRISPRi (dCas9-KRAB) under the control of doxycyline. iAT2s were transduced with a lentivirus expressing gRNA targeting the transcriptional start site of DSP. Cells were treated with or without doxycyline to intiate CRISPRi-knockdown. Prior to capture, cells were labelled with hashing antibodies (HTO). Cells were captured for 10x Genomics Single cell capture (V3 protocol), and GEX and HTO libraries were sequenced. HTODemux function was used to demultiplex the samples. Knockdown of DSP caused cells to cluster separately from wild-type cells.

2022-05-26 | GSE189068 | GEO

Single cell RNAseq of primary bronchial epithelial cells from healthy, ex-smoker COPD and current smoker COPD patients cultured in ALI conditions

Project description:Single cell RNAseq of primary bronchial epithelial cells from healthy, ex-smoker COPD and current smoker COPD patients cultured in ALI conditions

2026-04-29 | GSE302794 | GEO

Single cell ATACseq of primary bronchial epithelial cells from healthy, ex-smoker COPD and current smoker COPD patients cultured in ALI conditions

Project description:Single cell ATACseq of primary bronchial epithelial cells from healthy, ex-smoker COPD and current smoker COPD patients cultured in ALI conditions

2026-04-29 | GSE302411 | GEO

ATAC-seq analysis of primary bronchial epithelial cells from healthy, ex-smoker COPD and current smoker COPD patients cultured in ALI conditions

Project description:ATAC-seq analysis of primary bronchial epithelial cells from healthy, ex-smoker COPD and current smoker COPD patients cultured in ALI conditions

2026-04-29 | GSE302115 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data