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Lipid metabolizing enzymes differ between preputial gland sebocytes and sebaceous gland sebocytes

ABSTRACT: Lipid metabolizing enzymes differ between preputial gland sebocytes and sebaceous gland sebocytes

PROVIDER: PRJNA768427 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16194235.fastq.gz	Fastqsanger.gz
	SRR16194236.fastq.gz	Fastqsanger.gz
	SRR16194237.fastq.gz	Fastqsanger.gz
	SRR16194238.fastq.gz	Fastqsanger.gz
	SRR16194239.fastq.gz	Fastqsanger.gz

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Lipid metabolizing enzymes differ between preputial gland sebocytes and sebaceous gland sebocytes

Project description:Due to limited model systems for sebaceous glands we investigated potential suitability of preputial glands as model for sebaceous glands. We performed single-cell sequencing of murine preputial glands and back skin biopsies. This study revealed striking differences in lipid metabolizing enzyme expression and differentiation pathways found in sebocytes of the different tissues.

2022-01-24 | GSE185268 | GEO

Identification of genes involved in sebaceous gland carcinoma of the eyelid

Project description:Sebaceous gland carcinoma is the frequent malignant tumor of the eyelid. However, there is limited understanding of how altered gene expression of sebaceous gland carcinoma of the eyelid related to the pathogenesis. In this study, to identify genes involved in sebaceous gland carcinoma of the eyelid, global-scale gene expression analysis was carried out using a GeneChip® system.

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Effects of (-)-cannabidiol on human sebocytes

Project description:In the current study, we aimed at exploring the effects of the major non-psychotropic phytocannabinoid of Cannabis sativa, (-)-cannabidiol (CBD), on human sebaceous gland functions. We found that CBD behaved as a highly effective anti-acne agent, targeting all the three key, sebocyte-specific steps of the pathogenesis (i.e. without compromising viability or basal sebaceous lipid production, CBD normalized pro-acne agents-induced excessive lipid synthesis, reduced proliferation and exerted anti-inflammatory actions). The goal of the present microarray analyses was to identify signaling pathways and target genes being involved in mediating the above beneficial effects of CBD. Microarray analyses were performed by using three independent sets of control (vehicle-treated) and CBD-treated (10 M-NM-<M, 24-hr) human, immortalized SZ95 sebocytes. The first two sets of the samples were analyzed by two-color microarrays (M-bM-^@M-^\rep1M-bM-^@M-^] and M-bM-^@M-^\rep2M-bM-^@M-^]). Later on, the RNA from the second sample set was used for the M-bM-^@M-^\rep2-1M-bM-^@M-^] hybridization (techical repeat). Finally, we analyzed the third (biological) sample set by using one-color microarray (M-bM-^@M-^\rep3M-bM-^@M-^]).

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High-Resolution Spatial Map of the Human Facial Sebaceous Gland Reveals Marker Genes and Decodes Sebocyte Differentiation

Project description:The sebaceous gland is essential for skin homeostasis by producing sebum to lubricate and protect the skin. However, its cellular and molecular mechanisms in humans remain poorly understood as most studies have been conducted in mouse models. This study provides a comprehensive molecular analysis of the human sebaceous gland, focusing on cellular interactions, novel gene markers, and sebocyte differentiation. By integrating Stereo-seq spatial transcriptomics, single-cell RNA sequencing, and validation though MERFISH, we identified four distinct stages of sebocyte differentiation, each characterized by unique gene signatures. These results reveal sebocyte differentiation as a dynamic and complex process. Our findings enhance the understanding of sebaceous gland biology and provide a valuable reference for future research and the development of therapies for sebaceous gland-related disorders, including acne.

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High-Resolution Spatial Map of the Human Facial Sebaceous Gland Reveals Marker Genes and Decodes Sebocyte Differentiation [MERFISH]

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Elucidating the Cellular and Molecular Dynamics of Sebaceous Gland Self-Renewal and Regeneration

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Differentially expressed miRNAs for sebaceous gland carcinoma of the eyelid and TP53 mutation

Project description:To screen the related miRNAs in the occurence and development of sebaceous gland carcinoma(SGC) of the eyelid , we have employed Human Agilient microarray expression profiling as a discovery platform to identify differentially expressed miRNAs for SGC and TP53 mtation related miRNAs. Human formalin-fixed paraffin-embedded (FFPE) SGC tissues and para-carcinoma sebaceous gland(SG) FFPE samples from SGC patients were screened using microarray assay. Human FFPE SGC tissues containing TP53 mutation and human FFPE SGC tissues without TP53 mutation were screened using microarray. The overlap of two sets of microarray differentially upregulated miRNAs were selected as our research object.

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Project description:TRPV3 is highly expressed in human skin and is involved in the development of inflammatory dermatoses. However, it remains unclear whether TRPV3 influences inflammation in human sebaceous glands and its role in the pathogenesis of acne. Here, we showed that TRPV3 expression was increased in the sebaceous glands of facial acne lesions and acne-like mice. TRPV3 increased the secretion of pro-inflammatory cytokines and chemokines in human SZ95 sebocytes, as well as the chemotaxis of neutrophils, which were the major immune cells found in acne lesions. We demonstrated that P.acnes promoted TRPV3 expression through regulating lipid profile especially upregulated arachidonic acid levels in human sebocytes. TRPV3 further upregulated TLR2 expression by promoting transcriptional factor p-FOSL1 expression and its binding to the TLR2 promoter, leading to downstream NF-κB signaling activation. Importantly, either genetic silencing or pharmacological inhibition of TRPV3 alleviated acne-like inflammation in mice, showing reduced acne-characteristic cytokines and chemokines production and neutrophil infiltration by inhibiting the TLR2-NF-κB axis. Thus, our study revealed the critical role of TRPV3 in sebocytes inflammation, which was involved in the development of acne, indicating that TRPV3 is a potential therapeutic target for acne and other disorders of the pilosebaceous unit.

2023-10-13 | GSE245001 | GEO

SZ 95 sebocytes - OLR1 knockdown - treated with UV-oxidized PAPC

Project description:Human SZ95 sebocytes were transfected by lipofection with either scramble siRNA or OLR1 siRNA and after 24h were treated with 25µg/ml of UV-oxidized 1-palmitoyl-2-arachidonoyl-sn-phosphocholine (UVPAPC) for 7 hours. Phospholipid oxidation products (OxPL) are versatile stress signaling mediators in the skin. These lipid signaling molecules can be generated non-enzymatically or enzymatically by ultraviolet light, the major extrinsic skin aging factor. OxPL regulate cytoprotective, immunological and metabolic adaptation of the skin to oxidant stress. We here investigated whether the scavenger receptor Oxidized Low Density Lipoprotein Receptor 1 (OLR1, LOX-1) would have a function in cutaneous oxPL signaling. We found that OLR1 is expressed in several cutaneous cell types, most prominently in cells of the sebaceous gland and in keratinocytes. We repressed OLR1 expression with siRNA in SZ95 sebocytes, exposed cells to oxidized 1-palmitoyl-2-arachidonoyl-sn-phosphatiylcholine (PAPC) and performed transcriptomic profiling. Bioinformatic analysis revealed that OxPL exposure induced the Nrf2 antioxidant stress response and aldosterone signaling. The analysis also revealed that OLR1 is not required for the transcriptional regulation induced by oxidized PAPC, but interestingly, OLR1 knockdown affected expression of CNN2, HMRR, ITGB6 and KIF20A, all genes governing cell proliferation and motility. We identify sebocytes as cutaneous cells responsive to lipid mediated redox stress which is not dependent on the scavenger receptor OLR1.

2020-04-15 | GSE98335 | GEO

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