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Single nucleus transcriptomics: Apical resection in newborn pigs extends the time-window of cardiomyocyte proliferation and myocardial regeneration

ABSTRACT: Single nucleus transcriptomics: Apical resection in newborn pigs extends the time-window of cardiomyocyte proliferation and myocardial regeneration

PROVIDER: PRJNA768464 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16201500.fastq.gz	Fastqsanger.gz
	SRR16201600.fastq.gz	Fastqsanger.gz
	SRR16201501.fastq.gz	Fastqsanger.gz
	SRR16201601.fastq.gz	Fastqsanger.gz
	SRR16201502.fastq.gz	Fastqsanger.gz

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Single nucleus transcriptomics: Apical resection in newborn pigs extends the time-window of cardiomyocyte proliferation and myocardial regeneration

Project description:Background: In this study, we have extended the myocardial regenerative window to postnatal day 28 (P28) by a double injury pig model (ARP1MIP28) of apical resection at P1 (ARP1) and LAD ligation at P28 (MIP28). However, the molecular mechanism of regenerative window extension is not known. Results: Gene-expression profiles revealed that regenerating ARP1MIP28 hearts contained different subpopulations of cardiomyocytes early after 2nd injury. The six cardiomyocyte clusters were identified (CM1-CM6) by Louvain clustering. The CM6, CM3, and CM2 clusters have mainly consisted of fetal (92.4%), CTL-P1 (95.7%), and CTL-P56 (96.2%) cardiomyocytes. In contrast, the CM1 cluster has consisted of heterogeneous cardiomyocyte groups from all other animal groups, including less than 1% of fetal and CTL-P1 cardiomyocytes. The CM5 and CM4 clusters have mainly consisted of ARP1MIP28-P35 cardiomyocytes, 97.8%, and 68.6%, respectively. Sparse modeling analysis revealed that AR and MI injury, both alone and in combination, appeared to promote the proliferative capacity of cardiomyocytes and perturb cardiomyocyte maturation. Publication: This dataseries is associated to manuscript titled 'Single nucleus transcriptomics: Apical resection in newborn pigs extends the time-window of cardiomyocyte proliferation and myocardial regeneration', published in Circulation, 2021.

2021-10-06 | GSE185289 | GEO

Newborn apical resection preserves the proliferative capacity of cardiomyocytes located throughout the left ventricle

Project description:Newborn apical resection preserves the proliferative capacity of cardiomyocytes located throughout the left ventricle

| PRJNA1242376 | ENA

Single Nucleus Transcriptomics: Apical Resection in Newborn Pigs Extends the Time Window of Cardiomyocyte Proliferation and Myocardial Regeneration.

Project description: Not available

| S-EPMC9202233 | biostudies-literature

Newborn apical resection preserves the proliferative capacity of cardiomyocytes located throughout the left ventricle

Project description:Background: When pigs underwent apical resection (AR) on postnatal day (P) 1 (ARP1) followed by myocardial infarction (MI) on P28, the hearts had little evidence of scarring; meanwhile, hearts underwent MI on P28 without ARP1 showed large infarcts on P56; and the improvement of ARP1 hearts was driven primarily by cardiomyocyte proliferation. AR and MI were performed ~5 mm (AR) and ~20 mm (MI) above the heart apex; thus, we hypothesize that ARP1 preserved the cardiomyocytes cell-cycle throughout the left ventricle, rather than only near the resection site. Methods: Sections of cardiac tissue were collected from the left ventricle of uninjured pigs and from both the border zone (BZ) of AR and uninjured regions (remote zone, [RZ]) in ARP1 hearts. Cardiomyocyte proliferation was evaluated via immunofluorescence analysis of phosphorylated histone 3 [PH3] and symmetric Aurora B (sAuB). Single nucleus RNA sequencing (snRNAseq) data collected from the hearts of fetal pigs, uninjured pigs, and the BZ and RZ of ARP1 pigs was evaluated via our cell-cycle-specific Autoencoder to identify proliferating cardiomyocytes. Results: Cardiomyocyte PH3 and sAuB expression, and percentage of proliferating cardiomyocytes in snRNA data was significantly more common in both BZ and RZ of ARP1 than uninjured hearts but did not differ significantly between the ARP1-BZ and ARP1-RZ at any time point. Heat shock protein HSPA5 and HSP90B1 were overexpressed at both ARP1-BZ and ARP1-RZ. In AC16 cell, overexpression (and knockdown) of HSPA5-HSP90B1 increased (and decrease) cell-cycle activity. Conclusion: ARP1 preserved proliferative capacity of cardiomyocytes located throughout the left ventricle.

2025-03-27 | GSE293028 | GEO

Apical periodontitis

Project description:Apical periodontitis

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Expression data from neonatal heart after apex resection

Project description:Apical resection in 1-day-old mice results in complete regenerated heart and normal cardiac function, which is characterized by significant cardiomyocyte proliferation with minimal fibrosis. Local damage on hearts of 1-day-old mice can amplify cardiomyocyte proliferation located in the whole ventricle rather than limited damage area. This response provides a reasonable screening model to identify novel genes involved in endogenous cardiac regeneration

2020-07-10 | GSE154071 | GEO

newborn mice

Project description:newborn mice Raw sequence reads

| PRJNA1048552 | ENA

Microdissection: Transdifferentiation of Root Apical Meristem to Shoot Apical Meristem

Project description:gnp07_regeneome_transdifferenciation - microdissection - Study of the moleculars mecanism during transdifferenciation of Root ApicalMeristem to Shoot Apical Meristem - middle of growth permits to induce transdifferenciation of root apical meristem to shoot apical meristem

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Microdissection: Transdifferentiation of Root Apical Meristem to Shoot Apical Meristem

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RNA-seq from the hearts of 7-day-old mice that underwent apical resection and anti-arrhythmic drug treatment

Project description:RNA-seq from the hearts of 7-day-old mice that underwent apical resection and anti-arrhythmic drug treatment

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