Embryophyta
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA769194 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:DNA barcoding of plants in Hiroshima Prefecture, Japan
| PRJDB14597 | ENA
Project description:Organelle genomes of plants in Miyajima Island, Japan
| PRJDB14452 | ENA
Project description:Subcellular organization of RNAs and proteins is critical for cell function, but we still lack global maps and conceptual frameworks for how these molecules are localized in cells and tissues. Here we introduce ATLAS-Seq, which generates transcriptomes and proteomes from detergent-free tissue lysates fractionated across a sucrose gradient. Proteomic analysis of fractions confirmed separation of subcellular compartments. Unexpectedly, RNAs tended to co-sediment with other RNAs in similar protein complexes, cellular compartments, or biological functions. With the exception of those encoding secreted proteins, most RNAs sedimented differently than their encoded protein counterparts. To identify RNA binding proteins potentially driving these patterns, we correlated their sedimentation profiles to all RNAs, confirming known interactions and predicting new associations. Hundreds of alternative RNA isoforms exhibited distinct sedimentation patterns across the gradient, despite sharing most of their coding sequence. These observations suggest that transcriptomes can be organized into networks of co-segregating mRNAs encoding functionally related proteins, and provide insights into the establishment and maintenance of subcellular organization.
2020-04-22 | GSE140630 | GEO
Project description:Genome-wide analysis of the prmt5 and prmt4a;4b mutant transcriptomes
2019-05-14 | E-GEOD-62024 | ExpressionAtlas
Project description:Quantitative analysis of wild type and cd36-/- murine peritoneal macrophage transcriptomes
2020-04-29 | E-GEOD-139439 | ExpressionAtlas
Project description:Next Generation Sequencing Facilitates Quantitative Analysis of WT and dek42 mutant Transcriptomes
2020-04-06 | E-GEOD-118498 | ExpressionAtlas
Project description:Next Generation Sequencing Facilitates Quantitative Analysis of WT and zmbzip22 mutant Transcriptomes
2020-04-06 | E-GEOD-113048 | ExpressionAtlas
Project description:Next Generation Sequencing Facilitates Quantitative Analysis of WT and o11 mutant Transcriptomes
2020-04-06 | E-GEOD-97422 | ExpressionAtlas