Project description:This study investigates the genome-wide DNA-binding profile of the small regulatory protein NsrC in Synechococcus elongatus PCC 7942. Chromatin immunoprecipitation sequencing was performed using a strain expressing NsrC fused to mNeonGreen and FLAG (D1339). Two biological ChIP replicates and their matched input controls were analyzed to identify NsrC-associated genomic regions and support evaluation of its role in regulating phycobilisome biosynthesis genes. Intellectual property notice: The submitters claim intellectual property rights in certain aspects of this study. Users intending to publish analyses or file patent applications substantially based on these data are requested to contact the corresponding investigator, Guoyan Zhao (zhaoguoyan@sdnu.edu.cn), in advance. This notice does not restrict public access to the GEO/SRA records.
Project description:In order to explore the effect of introducing the ectoine synthesis module in Synechococcus elongatus PCC 7942 on the global regulation of the strain under high-salt environment. The ectoine synthesis module was introduced at the Synpcc7942_0808 locus to obtain the Syn7942/Δsps-ect strain that lacked sucrose synthesis and could synthesize ectoine. Comparative transcriptomic analysis was performed on WT and Syn7942/Δsps-ect under 0 mM and 300 mM NaCl conditions.
Project description:This RNA-seq study examines transcriptional responses of a cyanobacterium–yeast co-culture system comprising Synechococcus elongatus PCC 7942 and Rhodotorula toruloides NBRC 0880 (Rhodosporidium toruloides IFO0880) under multiple conditions. Experiments included planktonic co-cultures in 50 mL tubes subjected to chemical stressors (oxidative stress, photosynthesis inhibition), temperature stress, nutrient limitation (nitrogen, phosphorus), pH extremes, and heterotrophic conditions, as well as physically separated co-cultures in a 600 mL membrane bioreactor. Axenic controls for Synechococcus elongatus PCC 7942 were included for comparison. Differential gene expression analysis provides insight into interspecies interactions and individual organism responses across co-culture configurations.
