Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

COM33 reverses the expression of carboplatin-induced EMT and ERK signaling in SKOV3 cells

ABSTRACT: COM33 reverses the expression of carboplatin-induced EMT and ERK signaling in SKOV3 cells

PROVIDER: PRJNA770640 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR16299743_1.fastq.gz	Fastqsanger.gz
	SRR16299743_2.fastq.gz	Fastqsanger.gz
	SRR16299744_1.fastq.gz	Fastqsanger.gz
	SRR16299744_2.fastq.gz	Fastqsanger.gz
	SRR16299745_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

COM33 reverses the expression of carboplatin-induced EMT and ERK signaling in SKOV3 cells

Project description:The SKOV3 cells were treated with carboplatin or a combination of carboplatin and COM33 for 48h. The cells were lysed by TRIzol and total RNA was used for library construction. After cluster generation, the library preparations were sequenced on an Illumina HiSeq 4000 platform by Shanghai Life Genes Biotech Co. Ltd (Shanghai, China), and 150 bp paired-end reads were generated. After processing, filtering and checking quality, differentially expressed genes (DEGs) were screened using the following criteria: P < 0.05 and absolute log2 (fold change) > 1.0.

2024-10-12 | GSE185735 | GEO

RNAseq of SKOV3(AA) and SKOV3(CC)

Project description:To study the effect of rs1192691 on ovarian cancer cells, we generated SKOV3(CC) from SKOV3(A/A) using CRISPR/Cas9 technology and performed RNA-seq.

2022-01-18 | GSE181884 | GEO

RNAseq of SKOV3(AA) and SKOV3(CC)

Project description:RNAseq of SKOV3(AA) and SKOV3(CC)

| PRJNA753808 | ENA

Next Generation Sequencing of Transcriptomes in SKOV3 and rhCCL20-treated SKOV3 cells

Project description:The goals of this study are to compare the transcriptome differences between SKOV3 and rhCCL20-treated SKOV3 cells and identify the defferential expressed genes regulated by rhCCL20 in SKOV3 cells. We treated SKOV3 cells with 5% trehalose (control group) and rhCCL20 protein dissolved in 5% trehalose (experimental group) in vitro. The cells were collected 24 hours after treatment and used for RNA-sequencing.

2020-11-20 | GSE161799 | GEO

Erk-dependent Epigenetic Reprogramming Underlies Epithelial to Mesenchymal Transition

Project description:The series of events that allows for the conversion of adherent epithelial cells into migratory cells is collectively known as epithelial-mesenchymal transition (EMT). EMT is involved in triggering neural crest migration during development and in the pathogenesis of diseases, such as cancer metastasis. Whereas Erk signalling is known to be essential for EMT, its influence on the epigenetic and transcriptional programme underlying EMT is poorly understood. Here, using a comprehensive genome-wide analysis of H3K27ac mark and gene expression in primary mammary epithelial cells undergoing EMT, we found that Erk signalling is essential for the epigenetic reprogramming underlying hallmark gene expression and phenotypic changes of EMT. We found that the chemical inhibition of Erk signalling during EMT prevents loss and gain of H3K27ac mark at regulatory regions of epithelial and mesenchymal genes, respectively, and results in a transcriptome and epigenome closer to those of epithelial cells. Further computational analyses identified a distinct set of transcription factor motifs enriched at distal regulatory regions that are epigenetically remodelled by Erk signalling. Altogether, our study reveal an Erk-dependent epigenetic remodelling of distal regulatory elements that results in a gene expression programme that is essential for driving EMT.

2017-06-13 | GSE92333 | GEO

H3K27ac, H3K4me3, H3K27me3 and H3K9me3 ChIPseq of SKOV3(AA) and SKOV3(CC)

Project description:To study the effect of rs1192691 on histone modifications, we performed H3K27ac, H3K4me3, H3K27me3 and H3K9me3 in SKOV3(AA) and SKOV3(CC).

2022-01-18 | GSE182333 | GEO

The EBV-Encoded Oncoprotein, LMP1, Induces an Epithelial-to-Mesenchymal Transition (EMT) via its CTAR1 Domain Through Integrin-Mediated ERK-MAPK Signalling

Project description:The Epstein-Barr virus (EBV)-encoded LMP1 oncogene can induce profound effects on epithelial growth and differentiation including many of the features of the epithelial-to-mesenchymal transition (EMT). To better characterise these effects, we used the well-defined MDCK cell model and found that LMP1 expression in these cells induces EMT as defined by characteristic morphological changes accompanied by loss of E-cadherin, desmosomal cadherin and tight junction protein expression. The induction of the EMT phenotype required a functional CTAR1 domain of LMP1 and studies using pharmacological inhibitors revealed contributions from signalling pathways commonly induced by integrin-ligand interactions: ERK-MAPK, PI3-Kinase and tyrosine kinases, but not TGFbeta. More detailed analysis confirmed the role of integrin and ERK-MAPK signalling in the LMP1-induced EMT and implicated the CTAR1-mediated induction of Slug and Twist in these effects. A key role for beta1 integrin signalling in LMP1-mediated ERK-MAPK and FAK phosphorylation was observed and beta1 integrin activation was found to enhance LMP1-induced cell viability and survival. These findings support an important role for LMP1 in the early stages of NPC pathogenesis through transcriptional reprogramming that enhances tumour cell survival and leads to a more invasive, metastatic phenotype.

2018-06-06 | GSE111340 | GEO

A SIX1/EYA2 small molecule inhibitor disrupts EMT and metastasis

Project description:This study identified a novel compound reverses SIX1-mediated EMT and metastasis in breast cancer

2020-05-30 | GSE151491 | GEO

Gene Expression in Taxol Resistance SKOV3 Cells (primary)

Project description:To investigate the differences in gene expression between parental SKOV3 cells and the two taxol-resistant cell lines SKOV3/Tx50 and SKOV3/Tx600.

2015-07-16 | GSE60335 | GEO

Gene Expression in Taxol Resistance SKOV3 Cells (II)

Project description:To investigate the differences in gene expression between parental SKOV3 cells and the two taxol-resistant cell lines SKOV3/Tx50 and SKOV3/Tx600.

2015-07-16 | GSE58877 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data