Similar Datasets
Project description:We use ChIP-seq targeting histone 3 lysine 27-acetylation (H3K27ac) to identify putative enhancer sites genome-wide in the ventral pallidum cortex of adult prairie voles
2018-01-31 | GSE85826 | GEO
Project description:This study aimed to uncover changes in gene expression in key brain regions associated with social bonding neuroplasticity in adult prairie voles. The experimental workflow involved the following steps: 1. Male (Ma) and female (Fe) prairie voles were subjected to social cohabitation and mating (SCM) conditions for 120 h, with isolated animals serving as controls. 2. RNA was extracted and analyzing RNA from three brain regions crucial for social bonding and neuroplasticity: the subventricular zone (SVZ), dentate gyrus (DG), and nucleus accumbens (NAc). 3. RNA sequencing was performed to identify differentially expressed genes between the SCM and control groups. 4. Gene ontology (GO) and functional enrichment analyses were performed to understand the biological processes involved in pair bonding. 5. Validation of key findings through quantitative PCR and in vitro neurosphere assays using cells isolated from the SVZ. This approach allowed the examination of sex-specific changes in gene expression related to neurogenesis, synaptic plasticity, and other probable molecular pathways involved in pair bond formation, with potential implications for understanding human relationships and related neuropsychiatric conditions.
2025-05-15 | E-MTAB-15128 | biostudies-arrayexpress
Project description:An Infinium microarray platform (GPL28271, HorvathMammalMethylChip40) was used to generate DNA methylation data from several tissues from prairie voles (Microtus ochrogaster). Ear, liver, and brain samples from the Cornell University prairie vole colony were collected from 48 male and female prairie voles at various life stages: neonatal (<1 month old), sub-adult (2-4 months old), mature adult (4-10 months old), and middle aged/old adult (>10 months old). The pair bonded male and female prairie voles used in our study cohabitated with their partners for several months and produced at least three generations of litters. Animals were euthanized via rapid decapitation, their tissues rapidly extracted and frozen on dry ice before being stored at -80C until further processing for genomic DNA extraction. Brains were coronally sectioned and brain regions from the pair bonding circuit (PBC) were micro-dissected and pooled for each animal. The PBC brain regions included the prefrontal cortex, nucleus accumbens, lateral septum, ventral pallidum, and medial amygdala, and ventral tegmental area. Genomic DNA was isolated and purified using the phenol-chloroform extraction and ethanol precipitation method. A total of 144 tissue samples were collected and processed for DNA methylation analysis. Tissues: Brain, Ear, Liver
2023-08-07 | GSE174758 | GEO
Project description:Genetic structure, ecological versatility, and skull shape differentiation in Arvicola water voles
| PRJEB36026 | ENA
Project description:We compare fore- and mid-brain transcriptomes of reproductive males in monogamous and non-monogamous species pairs of Peromyscus mice, Microtus voles, parid songbirds, dendrobatid frogs, and Xenotilapia species of cichlid fishes. Our study provides evidence of a universal transcriptomic mechanism underlying the evolution of monogamy in vertebrates.
2018-12-18 | GSE123301 | GEO
Project description:Mammalian infants depend on parental care for survival, with numerous consequences for their behavioral development. We investigated the epigenetic and neurodevelopmental mechanisms mediating the impact of early parental care on social behavior development in prairie voles. We find widespread male specific differential expression of genes related to synaptic transmission and autism in the nucleus accumbens.
2023-07-31 | GSE229256 | GEO