Project description:4 groups of mice : Control, antibiotics, antibiotics + 4days of recolonization, antibiotics + 4days of recolonization + Enterocloster clostridioformis. Tumor draining lymph node were harvested after CFSE injection were harvested and CFSE+ cells were sorted and proccessed in order to generate single cell RNA-sequencing using BD Rhapsody mouse immune response targeted panel. Groups were barcoded using BD Rhapsody Multiplexing Kit.
Project description:To investigate the role of IRF4 in the regulation of ILC3 development and function, we used the wildtype mice as control and carried out single-cell RNA sequencing (BD Rhapsody) on gut ILC3s.
Project description:To investigate the role of aging in the regulation of ILC3 development and function, we used aged mice and carried out single-cell RNA sequencing (BD Rhapsody) on aged gut ILC3s.
Project description:To investigate the role of Cxxc1 in the regulation of ILC3 development and function, we used Cxxc1flox/floxRorccre mice and carried out single-cell RNA sequencing (BD Rhapsody) on small intestinal ILC3s.
Project description:To investigate the role of ThPOK in the regulation of ILC3 development and function, we established conventional ThPOK knockout mice and carried out single-cell RNA sequencing (BD Rhapsody) on intestinal ILC3s.
Project description:To investigate the role of aging in the regulation of ILC3 development and function, we used the young mice as control and carried out single-cell RNA sequencing (BD Rhapsody) on young gut ILC3s.
Project description:To investigate the role of IRF4 in the regulation of ILC3 development and function, we used the IRF4flox/floxRorccre mice and C57BL/6 mice(6-8 weeks-old ) to carry out single-cell RNA sequencing (BD Rhapsody) on gut ILC3s.
Project description:KLB-Cre mice were crossed to Ai14 mice to label KLB-expressing cells with tdTomato. The basolateral amygdala was then dissected out and tdTomato positive cells were isolated using FACs sorting. FACs sorted cells were then captured for single cell RNA sequencing analysis using the BD Rhapsody Whole Transcriptome Analysis kit.
Project description:We used targeted single cell RNA sequencing using a gene set composed of the BD Rhapsody Human Immune Response Panel, TCR panel, and a custom panel of 61 malignancy-associated genes and T/B cell receptor sequencing to characterize single cell populations in hypopigmented mycosis fungoides (HMF). Using a reference mapping model built on publically deposited classic mycosis fungoides single cell RNA sequencing datasets, we identified a predicted malignant cell population independent from the clonal T cell population in HMF.
