Project description:Parallel Analysis of RNA Ends (PARE) sequencing reads were generated to validate putative microRNAs and identify cleavage sites in Sorghum bicolor and Setaria viridis.
Project description:Transcript profiles of Laccaria bicolor S238N mycelium on various media were analyzed. The array probes were designed from gene models taken from the Joint Genome Institute (JGI, department of energy) Laccaria bicolor genome sequence version 1. One goal was to evaluate the effect of nutrient deprivation on the transcriptome of Laccaria bicolor.
Project description:Laccaria bicolor transcript profiles of different tissues and mycorrhizal root tips from different host trees were analyzed. The array probes were designed from gene models taken from the Joint Genome Institute (JGI, department of energy) Laccaria bicolor genome sequence version 1. One goal was to compare gene expression profiles from ectomycorrhizal root tips with different host plants.
Project description:The Transcriptome of different tissues and developmental stages of Laccaria bicolor S238N was analyzed. The array probes were designed from gene models taken from the Joint Genome institute Laccaria bicolor genome sequence version1. One aim of this study was to verify the expression of the automatically annotated gene models in various tissues and to use this transcriptional information to confirm, to correct or to reject gene models. Another goal was to identify tissue-specific gene expression, e.g. mycorrhiza up-regulated transcripts or fruiting body up-regulated transcripts, or treatment specific gene expression for further detailed analyses. Keywords: Tissue comparison
Project description:This study examines the proteomic responses in tissues of etiolated Sorghum bicolor BTx623 seedlings to salt stress (100 mM NaCl). The aim of the experiment is to investigate the induction and posssible role of Membrane Steroid Binding Protein 1 to salt tolerance in Sorghum.
Project description:Limonium bicolor, a typical recretohalophyte living in saline land, excretes excessive salt to the environment through salt glands in the epidermis for avoiding salt stress. The aim of this study was to screen genes involved in salt secretion by high-throughput RNA sequencing. A model was established to illustrate the candidate genes regulating salt secretion of salt gland. These genes will shed light on molecular mechanism of salt secretion of salt gland in plant.Normalized cDNA libraries of L. bicolor were constructed using mature leaves treated with 200 mM NaCl (with the highest salt secretion) and the control. Illumina paired-end platform was utilized to yield 2×100 bp independent reads. After de novo assembly, unigenes were aligned to the non-redundant (Nr) protein database and differentially expressed genes were enriched by GO annotations. Candidate genes were further verified by L. bicolor mutants with abnormal salt secretion.19,498 genes were targeted in Nr database and 5,768 were differentially expressed mapping to Arabidopsis, 2,269 up-regulated and 3,519 down-regulated under NaCl treatment compared with the control. Genes related to ion transport, vesicle, reactive oxygengen species scavenging, abscisic acid-dependent signal pathway and transcription factors were found high expression under NaCl treatment, of which 55 genes were likely involved in salt secretion and also confirmed by salt-secretion mutants.The present report identified candidate genes which are highly associated with salt secretion of L. bicolor salt gland. A salt transporting pathway was illustrated to explain how Na+ excreted outside by salt gland in L. bicolor. This data provides a useful reference source for salt secretion study of recretohalophytes.