Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chromatin accessibility of human memory T cell subsets from blood before and after ex-vivo CD3/CD28 stimulation

ABSTRACT: Chromatin accessibility of human memory T cell subsets from blood before and after ex-vivo CD3/CD28 stimulation

PROVIDER: PRJNA773945 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR16549331_1.fastq.gz	Fastqsanger.gz
	SRR16549331_2.fastq.gz	Fastqsanger.gz
	SRR16549332_1.fastq.gz	Fastqsanger.gz
	SRR16549332_2.fastq.gz	Fastqsanger.gz
	SRR16549333_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 112

Similar Datasets

Transcriptome analysis of human memory T cell subsets from blood before and after ex-vivo CD3/CD28 stimulation

Project description:Naive, central memory (TCM), effector memory (TEM), and terminally differentiated effector memory RA (TEMRA, CD8+ only) T cell subsets were FACS separated from PBMC samples of four human donors using CCR7 and CD45RA as distinguishing cell surface markers. Samples were split and either immediately isolated, or incubated for 42-48 hours with anti-CD3/CD28 beads for ex-vivo stimulation.

2023-03-01 | GSE186463 | GEO

Chromatin accessibility of human memory T cell subsets from blood before and after ex-vivo CD3/CD28 stimulation

2023-03-01 | GSE186462 | GEO

Transcriptome analysis of human memory T cell subsets from blood before and after ex-vivo CD3/CD28 stimulation

Project description:Transcriptome analysis of human memory T cell subsets from blood before and after ex-vivo CD3/CD28 stimulation

| PRJNA773946 | ENA

Creld1-deficient CD4+ T cells under homeostatic condition and CD3/CD28 stimulation

Project description:Transciptome profiling of sorted naive CD4+ T cells from control or Creld1 conditional KO mice (CD4-Cre) both directly ex vivo and with or without stimulation with anti-CD3/anti-CD28 antibodies to study the impact of Creld1 on T cell biology

2020-09-09 | GSE133863 | GEO

CD3/CD28 signaling, Jurkat E6.1, time course 27 mins

Project description:Fe-IMAC phosphoproteomics using TMT 11plex for quant, of Jurkat T cells stimulated with CD3 and CD28 agonist antibodies for 0, 3, 9, or 27 minutes.

| MSV000092965 | MassIVE

Methylation profiling of healthy human CD4+ cells after CD3 and CD28 stimulation

Project description:Genome-wide DNA methylation reference profiles of CD4+ cells after CD3 and CD28 stimulation in two healthy human individuals using the MeDIP technique a custom oligonucleotide array targetting the vast majority of annotated TSSs and non-promoter CGIs in the human genome.

2010-03-23 | E-MTAB-207 | biostudies-arrayexpress

Celiac disease T cell clone response to CD3/CD28 stimulation

Project description:To identify the CD4+ T cell cytokines responsible for the proliferation of the Lin-IEL lines CD4+ T cell clone L10, which recognises DQ2-glia-α1, one of the immunodominant T cell epitopes in celiac disease, was stimulated for 3 hours in IMDM with plate-bound CD3/CD28-specific (2.5 µg/ml each) or control antibodies coated onto 6-well non-tissue culture treated plates. Three independent biological replicates were performed, each time including 6 million Ficoll-purified live cells per condition. RNA was purified from these cells using the RNAeasy mini kit (Qiagen, Venlo, the Netherlands). cDNA was amplified using the Applause WT-Amp system (NuGEN technologies, Bemmel, the Netherlands) and biotin-labelled with the Encore Biotin Module (NuGEN). Human Gene 1.0 ST arrays (Affymetrix, High Wycombe, UK) were employed to quantify global gene expression.

2016-09-30 | GSE73599 | GEO

Genome wide expression profiles (RNAseq) of human CD8+ T cells in resting, activated (CD3+CD28) and PD-1-stimulated cells (CD3+CD28+PD-L1-Fc) at diferent time points

Project description:Background: Binding of the programmed death-1 (PD-1) receptor to its ligands (PD-L1/2) transduces inhibitory signals that promote exhaustion of activated T cells. Blockade of the PD 1 pathway is widely used for cancer treatment, yet the inhibitory signals transduced by PD-1 in T cells remain elusive. Methods: Expression profiles of human CD8+ T cells in resting, activated (CD3+CD28) and PD-1-stimulated cells (CD3+CD28+PD-L1-Fc) conditions were evaluated by RNA-seq. Bioinformatic analyses were used to identify signaling pathways differentially regulated in PD 1-stimulated cells.

2019-05-29 | GSE122149 | GEO

Creld1-deficient CD4+ T cells under homeostatic condition and CD3/CD28 stimulation

Project description:Creld1-deficient CD4+ T cells under homeostatic condition and CD3/CD28 stimulation

| PRJNA552845 | ENA

Gene expression in CD3/CD28 stimulated CD8 vs CD8 with various dosage of Cholesterol treatment

Project description:gene expression in in vitro diffrerentiated CD8 (CD3/CD28 stimulation ) T cells and CD8 T cells treated with Cholesterol

2020-04-14 | GSE112901 | GEO