Project description:Here, we used reverse-phase liquid chromatography-coupled tandem mass spectrometry to study the pre-weaned lamb proteome and metaproteome in ten different gastrointestinal tracts: rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum, colon, and rectum.
2017-12-14 | PXD008192 | Pride
Project description:lamb rumen fluid
| PRJNA831014 | ENA
Project description:Hu lamb rumen fluid
| PRJNA1140428 | ENA
Project description:Transcriptome of lamb rumen epithelium
Project description:Saccharina latissima is a brown alga commonly found in the North Atlantic, Arctic and Pacific oceans. Alginate constitutes a substantial portion of the cell wall of S. latissima, and its digestion requires a specific set of enzymes, alginate lyases. We investigated if and how S. latissima is metabolized in geographically distinct rumen ecosystems through in vivo lamb feeding experiments (2.5 and 5% inclusion, DM basis) and in vitro cattle-based rumen simulation technique, RUSITEC, experiments (up to 50% inclusion). Evidence supporting ruminal degradation of alginate was explored using a combination of 16S rRNA sequencing, metagenomics and metaproteomics, physiology (fluorescently labelled S. latissima hot water extracts (FLA-SLAT)) and biochemical characterization of PL6 alginate lyases.
2026-04-16 | PXD059090 | Pride
Project description:Transcriptome analysis of lamb rumen epithelium