Project description:Mosquitoes serve as medically significant vectors for transmitting various arboviruses. The mosquito midgut is both the digestive organ and primary infection site via viral bloodmeals, and the midgut cells show heterogeneity on virus-vector interactions. Rapidly developed single-cell RNA sequencing (scRNA-seq) technologies allow to characterize midgut physiology and viral infection dynamics at cellular solution. At present, scRNA-seq studies about mosquito midgut cell atlas are still limited and lack established protocols for cellular isolation. Therefore, we developed and employed a compatible microwell-based scRNA-seq protocol on four important mosquito species including Aedes aegypti, Aedes albopictus, Culex pipiens pallens and Culex tritaeniorhynchus and successfully established their midgut cell atlases pre- and post-bloodmeal. Further, we performed scRNA-seq experiments on DENV-infected Ae. aegypti to characterize the viral infection dynamics at single-cell solution.
Project description:Culex pipiens molestus and Cx. p. quinquefasciatus are the members of Culex pipiens Complex, but they display relatively large differences in behavior and physiological responses. We compared the genes of these mosquitoes to identify those that were differentially expressed in each subspecies. Such genes could play important roles in subspecies-specific blood feeding or oviposition behavior. Culex pipiens molestus and Cx. p. quinquefasciatus females were undertaken Illumina RNA sequencing.
Project description:Culex pipiens molestus and Cx. p. quinquefasciatus are the members of Culex pipiens Complex, but they display relatively large differences in behavior and physiological responses. We compared the genes of these mosquitoes to identify those that were differentially expressed in each subspecies. Such genes could play important roles in subspecies-specific blood feeding or oviposition behavior.
