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Environmental dependent heterosis and transgressive gene expression in reciprocal hybrids between the channel catfish Ictalurus punctatus and the blue catfish Ictalurus furcatus

ABSTRACT: Environmental dependent heterosis and transgressive gene expression in reciprocal hybrids between the channel catfish Ictalurus punctatus and the blue catfish Ictalurus furcatus

PROVIDER: PRJNA774773 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16609846_1.fastq.gz	Fastqsanger.gz
	SRR16609846_2.fastq.gz	Fastqsanger.gz
	SRR16609847_1.fastq.gz	Fastqsanger.gz
	SRR16609847_2.fastq.gz	Fastqsanger.gz
	SRR16609848_1.fastq.gz	Fastqsanger.gz

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Environmental dependent heterosis and transgressive gene expression in reciprocal hybrids between the channel catfish Ictalurus punctatus and the blue catfish Ictalurus furcatus

Project description:The hybrid between female channel catfish (Ictalurus punctatus) and male blue catfish (Ictalurus furcatus) is superior in feed conversion, disease resistance, carcass yield, and harvestability compared to both parental species. However, heterosis and heterobeltiosis only occur in pond culture, and channel catfish grow much faster than the other genetic types in small culture units. This environment-dependent heterosis is intriguing, but the underlying genetic mechanisms are not well understood. In this study, phenotypic characterization and transcriptomic analyses were performed in the channel catfish, blue catfish, and their reciprocal F1s reared in tanks. The results showed that the channel catfish is superior in growth-related morphometrics, presumably due to significantly lower innate immune function, as investigated by reduced lysozyme activity and alternative complement activity. RNA-seq analysis revealed that genes involved in fatty acid metabolism/transport are significantly upregulated in channel catfish compared to blue catfish and hybrids, which also contributes to the growth phenotype. Interestingly, hybrids have a 40-80% elevation in blood glucose than the parental species, which can be explained by a phenomenon called transgressive expression (overexpression/underexpression in F1s than the parental species). A total of 1,140 transgressive genes were identified in F1 hybrids, indicating that 8.5% of the transcriptome displayed transgressive expression. Transgressive genes upregulated in F1s are enriched for glycan degradation function, directly related to the increase in blood glucose level. This study is the first to explore molecular mechanisms of environment-dependent hetero-sis/heterobeltiosis in a vertebrate species and sheds light on the regulation and evolution of heterosis vs. hybrid incompatibility.

2022-01-05 | GSE186603 | GEO

Project description:Catfish farming is the largest aquaculture industry in the U.S., where hybrid catfish produced by channel catfish, Ictalurus punctatus ♀ × blue catfish, I. furcatus ♂, represent >50% of the harvest. Typically, older blue catfish (≥5 years) are selected for artificial fertilization. However, observations at our facilities and reports from hatcheries indicate a high degree of variation in reproductive performance among individuals. Thus, it is essential to establish links between paternal age and reproductive success, as it is apparent that advanced paternal age leads to changes in reproductive performance. Since there are no prior studies regarding the changes in testis transcriptome in blue catfish males during aging, we evaluated global gene expression of testis at age 2,4,7 and 9. Significant differential expressed genes and gene ontology of pairwise comparisons between age 2 and each test age are presented and discussed.

2025-03-17 | GSE290987 | GEO

Spermatogenesis, hormonal fluctuations, and testicular gene expression changes in male blue catfish, Ictalurus furcatus, throughout pubescent and early adult stages of development

Project description:Male blue catfish (Ictalurus furcatus) mature at 4-7 years and cannot be hand-stripped, making sperm collection a lethal procedure. Sperm are also highly variable in quality/quantity. Therefore, establishing links between seasonality, aging, and reproductive success is essential. Since there are no prior studies regarding seasonal and age-related changes in the testis transcriptome of male blue catfish, we evaluated global gene expression in testicular tissue over a 34-month period (2.3 to 4.9 years old; y/o). Significant differential expressed genes and gene ontology of pairwise comparisons are presented and discussed.

2025-08-09 | GSE304623 | GEO

Skin Deep: Early Mucosal Responses in Blue Catfish (Ictalurus furcatus) Skin to Aeromonas hydrophila Infection

Project description:Fish skin is a critical regulatory organ, serving not only as a physical barrier to pathogen entry, but also as a sophisticated integrator of aquatic environmental, social and nutritional cues through roles in immunity, osmoregulation, and endocrine signaling. Integral to the complexity of teleost skin is the mucus layer secreted by epidermal goblet cells. Pathogen invasion can disrupt this delicate homeostasis with profound impacts on signaling throughout the organism. Here, we investigated the transcriptional effects of virulent A. hydrophila infection in blue catfish skin, Ictalurus furcatus. We utilized an 8X60K Agilent microarray to examine gene expression profiles at critical early timepoints following challenge—2 h, 12 h, and 24 h. Expression of a total of 1,155 unique genes was significantly perturbed during at least one timepoint. We observed dysregulation of a number of genes involved in including antioxidant/apoptosis, cytoskeletal rearrangement, immune response, junctional/adhesion, and proteases. In particular, A. hydrophila infection rapidly altered a number potentially critical lectins, chemokines, interleukins, and other mucosal factors in a manner predicted to enhance its ability to adhere and invade the catfish host. Two-condition experiment, control vs. infected skin. Biological replicates: 3 control replicates, 3 infected replicates.3 timepoints

2014-06-17 | E-GEOD-42491 | biostudies-arrayexpress

Ictalurus furcatus

Project description:Ictalurus furcatus Transcriptome - Ictalurus furcatus.2

| PRJNA584794 | ENA

Ictalurus furcatus

Project description:Ictalurus furcatus

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Skin Deep: Early Mucosal Responses in Blue Catfish (Ictalurus furcatus) Skin to Aeromonas hydrophila Infection

2014-06-17 | GSE42491 | GEO

Ictalurus furcatus

Project description:Ictalurus furcatus overview

| PRJNA83703 | ENA

Environment-Dependent Heterosis and Transgressive Gene Expression in Reciprocal Hybrids between the Channel Catfish Ictalurus punctatus and the Blue Catfish Ictalurus furcatus.

Project description:The hybrid between female channel catfish (Ictalurus punctatus) and male blue catfish (Ictalurus furcatus) is superior in feed conversion, disease resistance, carcass yield, and harvestability compared to both parental species. However, heterosis and heterobeltiosis only occur in pond culture, and channel catfish grow much faster than the other genetic types in small culture units. This environment-dependent heterosis is intriguing, but the underlying genetic mechanisms are not well understood. In this study, phenotypic characterization and transcriptomic analyses were performed in the channel catfish, blue catfish, and their reciprocal F1s reared in tanks. The results showed that the channel catfish is superior in growth-related morphometrics, presumably due to significantly lower innate immune function, as investigated by reduced lysozyme activity and alternative complement activity. RNA-seq analysis revealed that genes involved in fatty acid metabolism/transport are significantly upregulated in channel catfish compared to blue catfish and hybrids, which also contributes to the growth phenotype. Interestingly, hybrids have a 40-80% elevation in blood glucose than the parental species, which can be explained by a phenomenon called transgressive expression (overexpression/underexpression in F1s than the parental species). A total of 1140 transgressive genes were identified in F1 hybrids, indicating that 8.5% of the transcriptome displayed transgressive expression. Transgressive genes upregulated in F1s are enriched for glycan degradation function, directly related to the increase in blood glucose level. This study is the first to explore molecular mechanisms of environment-dependent heterosis/heterobeltiosis in a vertebrate species and sheds light on the regulation and evolution of heterosis vs. hybrid incompatibility.

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Ictalurus furcatus

Project description:Ictalurus furcatus Transcriptome - Ictfu_L3

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