Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: RNA-seq of LCN2 stimulated HaCaT cells

PROVIDER: PRJNA775979 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR16611617_1.fastq.gz	Fastqsanger.gz
	SRR16611617_2.fastq.gz	Fastqsanger.gz
	SRR16611618_1.fastq.gz	Fastqsanger.gz
	SRR16611618_2.fastq.gz	Fastqsanger.gz
	SRR16611619_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Transcriptional responses by human skin cells to high-voltage electric field: Sham vs. SE-14000 stimulation

Project description:Transcriptional profiling of SE-14000 stimulated wound healing associated cells (HaCat, NHDF, HMEC-1) comparing sham-treated cells. Goal was to determine the effects of SE-14000 in the human skin cells (HaCat, NHDF, HMEC-1).

2021-02-19 | GSE110830 | GEO

Wound healing related cells: Control vs. SE-14000 stimulation

Project description:Transcriptional profiling of SE-14000 stimulated wound healing associated cells (HaCat, NHDF, HMEC-1) comparing control untreated cells. Goal was to determine the effects of SE-14000 to the wound healing associated cells (HaCat, NHDF, HMEC-1).

2020-07-07 | GSE100902 | GEO

The effects of terbutaline or GW9508 on TNF-alpha and IFN gamma (TNF-alpha + IFN gamma) stimulation by HaCaT

Project description:G-protein coupled receptors (GPCRs) have diverse roles in physiological processes, including immunity. Gs-coupled GPCRs increase while Gi-coupled ones decrease intracellular cAMP. Previous studies suggest that, in epithelial cells, Gs-coupled GPCRs enhance whereas Gi-coupled GPCRs suppress pro-inflammatory immune responses. In order to examine the issue, we chose beta2 adrenergic receptor and GPR40 as representatives of Gs- and Gi- coupled GPCRs, respectively, and examined their effects on TNF-alpha and IFN-gamma-(TNF-alpha + IFN-gamma) induced gene expression by HaCaT. We used microarrays to detail the global changes of gene expression induced by a beta2 adrenergic receptor agonist terbutaline or GPR40 agonist GW9508 pre-treatment in TNF-alpha + IFN-gamma - stimulated HaCaT cells. HaCaT cells were pre-treated with terbutaline or GW9508, TNF-alpha + IFN-gamma were then added, and cultured for another 24 h. Cells were then used for RNA extraction and hybridization on Affymetrix microarrays. We sought to clarify changes in gene expression after 1) TNF-alpha + IFN-gamma, 2) TNF-alpha + IFN-gamma + terbutaline, and 3) TNF-alpha + IFN-gamma + GW9508 treatment. To this end, we set 4 groups of samples; 1) unstimulated group, 2) TNF-alpha + IFN-gamma-stimulated group, 3) TNF-alpha + IFN-gamma + terbutaline-stimulated group, and 4) TNF-alpha + IFN-gamma + GW9508-stimulated group. In each group, HaCaT cells were stimulated in triplicate wells (n=3).

2010-12-01 | E-GEOD-20297 | biostudies-arrayexpress

Project description:RNA-seq of SREBP2-overexpressed HaCaT cells

| PRJNA776347 | ENA

Study of transcriptional effects of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line.

Project description:Aim of the study was to characterize at a molecular level (changes in transcriptomes) the effect of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line. This was adressed by using a culture model. The HaCaT cell line (human keratinocytes) was stimulated by MSU (1mg/mL) vs control for 12 hrs. By using genome-wide expression profiling, we identified deregulation of functionally relevant gene networks. HaCaT were obtained from Cell Lines Service (Eppelheim, Germany) and grown in DMEM medium (PAN biotech, Aidenbach, Germany) supplemented with 10% FBS (Life Technology, Grand Island, NY, USA), L-glutamine and non-essential amino acid. Before the treatment HaCaT cells were cultured in serum-free medium for 12hrs. HaCaT were treated with MSU (1mg/ml) vs DMEM control for 12hrs then submitted to RNA extration and gene expression profiling. Triplicate experiments were performed: HaCaT control (n=3), MSU-treated (n=3).

2015-10-29 | E-GEOD-65931 | biostudies-arrayexpress

RNA-seq in human keratinocytes HaCaT cell line

Project description:To figure out the expression level of genes in normal human keratinocytes, we performed RNA-seq analysis in HaCaT cells. Here, we used 4 HaCaT cells as parallel samples.

2023-01-04 | GSE202683 | GEO

Homo sapiens

Project description:Expression data from HaCaT cells stimulated by IL-22

| PRJNA293946 | ENA

Gene expression profile at single cell level of tumoral immune cells sorted from MB49 tumor tissues of wt and Lcn2 ko chimeric mice

Project description:Lcn2 was found as a marker of senescent neutrophils and to affect the tumor growth in our research. We used single cell RNA sequencing (scRNA-seq) to analyze the immune cells diversity and function change with Lcn2 knock-out

2025-02-24 | GSE287682 | GEO

Project description:RNA-Seq of HaCaT or HaCaT PPT1 KO cells after 20 Gy iradiation

| PRJNA1080437 | ENA

Effect of recombinant murine Lipocalin-2 (LCN2) on murine macrophages

Project description:We analyzed if LCN2 modulates the activity of phosphotyrosine kinases in LPS/IFNg-stimulated primary murine macrophages (BMDMs)

2024-02-18 | GSE225341 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data