Project description:Reference genome was assembed for mixture sample of Eriocheir sinensis eyestalk under low tempeture (7°C,12°C,17°C) ,normal temperature (22°C) and high temperature (27°C, 32°C) conditions.The reference genome was used for the further transcritome analysis of genes expression profiels in eystalk of Eriocheir sinensis under different tempetures and provide key molecular targets for elucidating the mechanisms by which temperature affects molting and development in E. sinensis.
Project description:The transcriptome profiling five tissues of juvenile Eriocheir sinensis, including gill, muscle, thoracic ganglion, eyestalk and hepatopancreas, were sequenced to get the basic dataset for constructed a genome-scale metabolic network model for E. sinensis. The model was used to predict the optimal nutrient requirements of E. sinensis in feed and suggestions for feed improvement were put forward based on the simulation results.
