Project description:The emergence of ciprofloxacin-resistant Salmonella poses a significant challenge to the development of new drugs. Probiotics that inhibit ciprofloxacin-resistant Salmonella offer a safer alternative with fewer side effects than chemical antibiotics and drugs. Here, a strain of Companilactobacillus alimentarius Y4, isolated from kimchi, demonstrated strong antibacterial activity against Salmonella C1, E. coli and other intestinal pathogens. The results of the biological characteristics experiment show that the survival rate of C. alimentarius Y4 was 80% at pH 3.0 and 87.13% at 0.3% bile salt concentration. It retained antibacterial activity even after exposure to pepsin, trypsin, protease K, and catalase. Additionally, C. alimentarius Y4 down-regulates the inflammatory cytokines IL-1β, TNF-α, and IL-6 while upregulating the anti-inflammatory cytokine IL-10. It effectively repaired intestinal damage caused by ciprofloxacin-resistant Salmonella C1. Gut flora analysis revealed that C. alimentarius Y4 colonized the intestine and increased the abundance of beneficial gut microbes such Lachnospiraceae bacterium-COE1 and Muribaculaceae-CAG-485. Furthermore,analysis of intestinal metabolites indicated that C. alimentarius Y4 Up-regulate L-leucine, L-lysine and Tryptophan and other metabolites and then restored intestinal metabolic balance. In summary, C. alimentarius Y4 by regulating inflammatory cytokines, repairing intestinal damage, rebalancing gut flora, and correcting intestinal metabolic disorders,it is holds promise as an effective probiotic for managing intestinal inflammation caused by ciprofloxacin-resistant Salmonella. This study lays a foundation for the clinical development of probiotic products.
Project description:To investigate the regulatory role of MLO-Y4 mitochondria on chondrocytes, we isolated mitochondria from MLO-Y4 and transplanted to chondrocytes. We then performed gene expression profiling analysis using data obtained from RNA-seq of chondrocytes from vehicle group and mitochondria transplanted group.
