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Next Generation Sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of microRNA miR-30e

ABSTRACT: Next Generation Sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of microRNA miR-30e

PROVIDER: PRJNA778407 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16832237_1.fastq.gz	Fastqsanger.gz
	SRR16832237_2.fastq.gz	Fastqsanger.gz
	SRR16832238_1.fastq.gz	Fastqsanger.gz
	SRR16832238_2.fastq.gz	Fastqsanger.gz
	SRR16832239_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of microRNA miR-30e

Project description:MicroRNAs (miRNAs) function as regulators of cancer progression as they modulate different cellular processes. The objective of this study is to demonstrate a multi-dimensional function of miR-30e through regulating genes involved in various signaling pathways including androgen receptor signaling. miR-30e targets androgen receptor mRNA and functions as a tumor suppressor. We noted loss of miR-30e expression in prostate tumors and restored expression of miR-30e led to cell cycle arrest, induction of apoptosis, improved drug sensitivity in prostate cancer cells, and reduced tumor progression in prostate cancer xenograft models. To understand the tumor suppressor function of miR-30e on a global scale, we performed total RNA-sequence analysis upon overexpression of miR-30e in a prostate cancer cell line 22Rv-1, which expresses a splice variant of androgen receptor and represents an androgen-independent and drug-resistant form of prostate cancer.qRT-PCR validation of RNA-sequencing data supports our in vitro study showeing differential expression of genes involved in cell cycle progression, apoptosis and ubiquitination upon expression of miR-30e in prostate cancer cells.

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Project description:This experiment is designed to evaluate gene expression alteration following miR-30e* transfection in glioma cells. We find a significant elevation of NF kappa B transcriptional targets.

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Project description:MiR-30e represses the osteogenic program in mesenchymal stem cells (MSCs) and smooth muscle cells (SMCs) by targeting IGF2, and drives their differentiation into adipogenic or smooth muscle lineage, respectively. In Experiment 1, SMCs were cultured from mouse aortas and transduced with miR-30e or control lentivirus. In Experiment 2, MSCs were extracted from mouse bone marrow and transduced with miR-30e or control lentivirus. In Experiment 3, the MSCs were transfected with a scrambled oligo or anti-miR-30e oligo. In all 3 experiments, N=3 per group. RNA was extracted from each experiment and run on Affymetrix arrays.

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Next Generation Sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of miR-5100

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