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Next Generation Sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of microRNA miR-30e

ABSTRACT: Next Generation Sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of microRNA miR-30e

PROVIDER: PRJNA778407 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16832237_1.fastq.gz	Fastqsanger.gz
	SRR16832237_2.fastq.gz	Fastqsanger.gz
	SRR16832238_1.fastq.gz	Fastqsanger.gz
	SRR16832238_2.fastq.gz	Fastqsanger.gz
	SRR16832239_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of microRNA miR-30e

Project description:MicroRNAs (miRNAs) function as regulators of cancer progression as they modulate different cellular processes. The objective of this study is to demonstrate a multi-dimensional function of miR-30e through regulating genes involved in various signaling pathways including androgen receptor signaling. miR-30e targets androgen receptor mRNA and functions as a tumor suppressor. We noted loss of miR-30e expression in prostate tumors and restored expression of miR-30e led to cell cycle arrest, induction of apoptosis, improved drug sensitivity in prostate cancer cells, and reduced tumor progression in prostate cancer xenograft models. To understand the tumor suppressor function of miR-30e on a global scale, we performed total RNA-sequence analysis upon overexpression of miR-30e in a prostate cancer cell line 22Rv-1, which expresses a splice variant of androgen receptor and represents an androgen-independent and drug-resistant form of prostate cancer.qRT-PCR validation of RNA-sequencing data supports our in vitro study showeing differential expression of genes involved in cell cycle progression, apoptosis and ubiquitination upon expression of miR-30e in prostate cancer cells.

2022-08-31 | GSE188345 | GEO

Next Generation Sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of miR-5100

Project description:Next Generation Sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of miR-5100

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Next-generation sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of LINC00888

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Next Generation Sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of miR-5100

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Next-generation sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of LINC00888

Project description:Transcriptome analysis was performed to determine the effect of overexpression of LINC00888 on global gene expression in prostate cancer cells.

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Proteomics of ectopic endometrial cell-derived exosomes in endometriosis

Project description:We primary cultured ectopic endometrial cells from patients with endometriosis (2 cases) and without endometriosis(2 cases) and collected cell culture supernatants（P0). We isolated exosomes from cell culture supernatants by differential centrifugation and then performed proteome analysis on the two groups of exosomes to investigate the role of ectopic endometrial cell-derived exosomes in the development of endometriosis.

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Circulating microRNA miR-323-3p as a biomarker of ectopic pregnancy.

Project description:BACKGROUND:The use of serum human chorionic gonadotropin (hCG) and progesterone to identify patients with ectopic pregnancy (EP) has been shown to have poor clinical utility. Pregnancy-associated circulating microRNAs (miRNAs) have been proposed as potential biomarkers for the diagnosis of pregnancy-associated complications. This proof-of-concept study examined the diagnostic accuracy of various miRNAs to detect EP in an emergency department (ED) setting. METHODS:This study was a retrospective case-control analysis of 89 women who presented to the ED with vaginal bleeding and/or abdominal pain/cramping and received a diagnosis of viable intrauterine pregnancy (VIP), spontaneous abortion (SA), or EP. Serum hCG and progesterone concentrations were measured by immunoassays. The serum concentrations of miRNAs miR-323-3p, miR-517a, miR-519d, and miR-525-3p were measured with TaqMan real-time PCR. Statistical analysis was performed to determine the clinical utility of these biomarkers, both as single markers and as multimarker panels for EP. RESULTS:Concentrations of serum hCG, progesterone, miR-517a, miR-519d, and miR-525-3p were significantly lower in EP and SA cases than in VIP cases (P < 0.01). In contrast, the concentration of miR-323-3p was significantly increased in EP cases, compared with SA and VIP cases (P < 0.01). As a single marker, miR-323-3p had the highest sensitivity of 37.0% (at a fixed specificity of 90%). In comparison, the combined panel of hCG, progesterone, and miR-323-3p yielded the highest sensitivity (77.8%, at a fixed specificity of 90%). A stepwise analysis that used hCG first, added progesterone, and then added miR-323-3p yielded a 96.3% sensitivity and a 72.6% specificity. CONCLUSIONS:Pregnancy-associated miRNAs, especially miR-323-3p, added substantial diagnostic accuracy to a panel including hCG and progesterone for the diagnosis of EP.

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MiR-30e in Aortic Smooth Muscle Cells and Bone Marrow Mesenchymal Stem Cells [Experiment 2]

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MiR-30e in Aortic Smooth Muscle Cells and Bone Marrow Mesenchymal Stem Cells [Experiment 1]

2015-01-30 | E-GEOD-65431 | biostudies-arrayexpress