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Double-stranded RNA formation leads to preferential nuclear export and gene expression

ABSTRACT: Double-stranded RNA formation leads to preferential nuclear export and gene expression

PROVIDER: PRJNA778861 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16883151.fastq.gz	Fastqsanger.gz
	SRR16883152.fastq.gz	Fastqsanger.gz
	SRR16883153.fastq.gz	Fastqsanger.gz
	SRR16883154.fastq.gz	Fastqsanger.gz
	SRR16883155.fastq.gz	Fastqsanger.gz

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Double stranded RNA formation leads to preferential nuclear export and gene expression

Project description:Double stranded RNA formation leads to preferential nuclear export and gene expression

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Double stranded RNA formation leads to preferential nuclear export and gene expression

Project description:mRNAs are transcribed and processed in the nucleus before they are exported into the cytoplasm for translation. Export is mediated by the export receptor heterodimer Mex67-Mtr2 in yeast (TAP-p15 in humans). Interestingly, also many lncRNAs leave the nucleus but it is currently unclear why they travel into the cytoplasm. Here we show that antisense (as)RNAs accelerate mRNA export by annealing with their sense counterparts through the helicase Dbp2. These double-stranded (ds)RNAs dominate export compared to single-stranded (ss)RNA, as they have a higher capacity and affinity for the export receptor Mex67. In this way, asRNAs boost gene expression, which is beneficial for cells. This is particularly important upon expression program changes. Consequently, the degradation or prevention of the formation of dsRNA is toxic for cells. This mechanism illuminates the general cellular occurrence of asRNAs and explains their nuclear export.

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Project description:mRNAs are transcribed and processed in the nucleus before they are exported into the cytoplasm for translation. Export is mediated by the export receptor heterodimer Mex67-Mtr2 in yeast (TAP-p10 in humans). Interestingly, also long non-coding RNAs (lncRNAs) leave the nucleus and so-called XUTs (Xrn1 sensitive unstable transcripts) accumulate in the cytoplasm of the degradation defective xrn1∆ mutant. Curiously, it is currently unclear why so many lncRNAs travel into the cytoplasm. Here we show that such ncRNAs accelerate mRNA export by annealing with their sense counterparts through the helicase Dbp2. These double stranded (ds) RNAs dominate export, because Mex67 has a higher affinity to dsRNAs and more molecules bind. Cells benefit from this mechanism as it quickly regulates gene expression, which is particularly important upon expression program changes. Consequently, prevention of the dsRNA formation or destroying dsRNAs is lethal to cells. This mechanism could explain the general cellular occurrence of antisense RNAs.

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