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Endothelial dysfunction in NR2F2-silenced cells - human lung microvascular endothelial cells model

ABSTRACT: Endothelial dysfunction in NR2F2-silenced cells - human lung microvascular endothelial cells model

PROVIDER: PRJNA779016 | ENA |

REPOSITORIES: ENA

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Endothelial dysfunction in NR2F2-silenced cells - human lung microvascular endothelial cells model

Project description:Orphan nuclear receptor chicken ovalbumin upstream promoter transcription factor 2 (COUPTF2; NR2F2) is highly expressed in endothelial cells (ECs) and Nr2f2 knockout produces lethal cardiovascular defects. In humans, NR2F2 mutations result in both congenital heart disease and diaphragmatic hernia, conditions associated with the development of pulmonary arterial hypertension (PAH). However, COUPTF2 functions in mature endothelium are uncertain. NR2F2 knockdown in primary human endothelial cells (ECs) led to an interferon-biased inflammatory response, endothelial-to-mesenchymal transition, proliferation, hypermigration, apoptosis-resistance and mitochondrial dysfunction. These phenotypic changes were associated with AKT activation and increased Dickkopf-1 (DKK1) expression, a Wnt/β-catenin pathway inhibitor. DKK1 was also elevated in patients with PAH and secreted in response to loss of bone morphogenetic receptor type 2 (BMPR2), the archetypal PAH-associated genetic defect. Together, these findings demonstrate that endothelial NR2F2 suppresses inflammation and proliferation. Thus, NR2F2 loss disrupts EC homeostasis and may promote pathologic vascular remodeling in the development of PAH.

2023-05-31 | GSE188467 | GEO

Endothelial dysfunction in NR2F2-silenced cells - EA.hy926 cells model

Project description:Endothelial dysfunction in NR2F2-silenced cells - EA.hy926 cells model

| PRJNA779018 | ENA

Endothelial dysfunction in NR2F2-silenced cells - EA.hy926 cells model

2023-05-31 | GSE188465 | GEO

Endothelial dysfunction in NR2F2-silenced cells - human coronary artery endothelial cells model

Project description:Endothelial dysfunction in NR2F2-silenced cells - human coronary artery endothelial cells model

| PRJNA779017 | ENA

Endothelial dysfunction in NR2F2-silenced cells - human coronary artery endothelial cells model

2023-05-31 | GSE188466 | GEO

Microvascular endothelial cells

Project description:Analysis of human microvascular endothelial cells derived from adipose tissue

2021-03-03 | MSV000086982 | MassIVE

RNAi profiling of human lymphatic endothelial cells after knockdown of Prox1 and/or NR2F2

Project description:Gene expression profiles of primary lymphatic endothelial cells (LECs) isolated from human foreskin were analyzed after siRNA-mediated knockdown of control (firefly luciferase), Prox1, NR2F2 or Prox1/NR2F2 for 48 hours. Experiment Overall Design: Passage five human lymphatic endothelial cells (LECs) were cultured on fibronectin (10 Î¼g/ml)-coated plates in a complete media (EBM, 20% FBS supplemented with 10 Î¼g/ml hydrocortisone acetate, 25 ug/ml cAMP and antibiotics). LECs were harvested and electorporated with siRNA duplexes for 48 hours with siRNA duplexes against either firefly luciferase(control), Prox1, NR2F2 or Prox1/NR2F2. Total RNA was purified using Tri-reagent and was subjected to microarray analysis. Experiment Overall Design:

2008-10-25 | E-GEOD-12846 | biostudies-arrayexpress

RNAseq of DHX15-silenced endothelial cells

Project description:RNAseq of DHX15-silenced endothelial cells

| PRJNA759857 | ENA

Gene expression profiles of lymphatic endothelial cells after knockdown of Prox1 and/or NR2F2

2008-09-20 | GSE12846 | GEO

Angio-modulation in endothelial cells: an unexpected role of desmoglein-2 in regulating actin dynamics and its relevance to angiogenesis deregulation in systemic sclerosis

Project description:Gene expression study of DSG2 silenced human microvascular endothelial cells Affymetrix GeneChip Human Genome U133 Plus 2.0 Array were used to examine differentially expressed genes between normal microvascular endothelial cells and DSG2 silenced microvascular endothelial cells

2012-10-01 | E-GEOD-21547 | biostudies-arrayexpress

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