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The RNA-binding proteins TIA1 and TIAL1 are required for the expression of the DNA damage repair machinery during B cell lymphopoiesis

ABSTRACT: The RNA-binding proteins TIA1 and TIAL1 are required for the expression of the DNA damage repair machinery during B cell lymphopoiesis

PROVIDER: PRJNA779409 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16904800_1.fastq.gz	Fastqsanger.gz
	SRR16904800_2.fastq.gz	Fastqsanger.gz
	SRR16904801_1.fastq.gz	Fastqsanger.gz
	SRR16904801_2.fastq.gz	Fastqsanger.gz
	SRR16904802_1.fastq.gz	Fastqsanger.gz

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The RNA-binding proteins TIA1 and TIAL1 are required for the expression of the DNA damage repair machinery during B cell lymphopoiesis

Project description:B-cell lymphopoiesis requires dynamic modulation of the B-cell transcriptome at the post-transcriptional level, although the implication of RNA-binding proteins (RBPs) remain largely unknown. Here we show that the RBPs TIA1 and TIAL1 are essential in B cells and, if deleted, there is a developmental block at the pro-B cell stage. TIA1 and TIAL1 have redundant functions. They act together as global splicing regulators for the expression of mRNAs including those involved in DNA damage repair in pro-B cells. Mechanistically, TIA1 and TIAL1 bind to 5’splice sites for exon definition, splicing and expression of DNA damage sensors like Chek2 and Rif1. In their absence, pro-B cells show exacerbated DNA damage, altered P53 expression and increased cell death. Altogether, our study uncovers the importance of tight regulation of mRNA splicing by TIA1 and TIAL1 for the expression of integrative transcriptional programs for DNA damage sensing and repair during B-cell development.

2022-12-20 | GSE188556 | GEO

The RNA-binding proteins TIA1 and TIAL1 are required for the expression of the DNA damage repair machinery during B cell lymphopoiesis.

Project description:B-cell lymphopoiesis requires dynamic modulation of the B-cell transcriptome at the post-transcriptional level, although the implication of RNA binding proteins (RBPs) remain largely unknown. Here we show that the RBPs TIA1 and TIAL1 are essential in B cells and, if deleted, there is a developmental block at the pro-B cell stage. TIA1 and TIAL1 have redundant functions. They act together as global splicing regulators for the expression of mRNAs including those involved in DNA damage repair in pro-B cells. Mechanistically, TIA1 and TIAL1 bind to 5’splice sites for exon definition, splicing and expression of DNA damage sensors like Chek2 and Rif1. In their absence, pro-B cells show exacerbated DNA damage, altered P53 expression and increased cell death. Altogether, our study uncovers the importance of tight regulation of mRNA splicing by TIA1 and TIAL1 for the expression of integrative transcriptional programs for DNA damage sensing and repair during B-cell development.

2022-12-20 | GSE186701 | GEO

The RNA-binding proteins TIA1 and TIAL1 are required for the expression of the DNA damage repair machinery during B cell lymphopoiesis.

Project description:The RNA-binding proteins TIA1 and TIAL1 are required for the expression of the DNA damage repair machinery during B cell lymphopoiesis.

| PRJNA775391 | ENA

The RNA binding proteins TIA1 and TIAL1 protect germinal centre responses from apoptosis

Project description:Germinal centres (GC) are essential for the establishment of long-lasting antibody responses. In there, GC B cells rely on post-transcriptional RNA mechanisms for translating activation-associated transcriptional programs into functional changes in the cell proteome. However, we still lack knowledge about which are the critical proteins driving these key mechanisms. Here we show that the RNA binding proteins TIA1 and TIAL1 are required for the generation of long-lasting GC responses. TIA1 and TIAL1- deficient GC B cells fail to undergo antigen- mediated positive selection, expansion and differentiation into B cell clones producing high-affinity antibodies. Mechanistically, TIA1 and TIAL1 control the transcriptional identity of dark and light zone GC B cells and enable timely expression of the pro-survival molecule MCL1. Altogether, we demonstrate here that TIA1 and TIAL1 are key players in the post-transcriptional program that selects high-affinity antigen-specific GC B cells.

2023-08-29 | GSE235358 | GEO

The RNA binding proteins TIA1 and TIAL1 protect germinal centre responses from apoptosis [iCLIP]

Project description:Germinal centres (GC) are essential for the establishment of long-lasting antibody responses. In there, GC B cells rely on post-transcriptional RNA mechanisms for translating activation-associated transcriptional programs into functional changes in the cell proteome. However, we still lack knowledge about which are the critical proteins driving these key mechanisms. Here we show that the RNA binding proteins TIA1 and TIAL1 are required for the generation of long-lasting GC responses. TIA1 and TIAL1- deficient GC B cells fail to undergo antigen-mediated positive selection, expansion and differentiation into B cell clones producing high-affinity antibodies. Mechanistically, TIA1 and TIAL1 control the transcriptional identity of dark and light zone GC B cells and enable timely expression of the pro-survival molecule MCL1. Altogether, we demonstrate here that TIA1 and TIAL1 are key players in the post-transcriptional program that selects high-affinity antigen-specific GC B cells.

2023-08-29 | GSE235655 | GEO

The splicing regulators TIA1 and TIAL1 are required for the expression of the DNA damage repair machinery during B cell lymphopoiesis.

Project description:B cell lymphopoiesis requires dynamic modulation of the B cell transcriptome for timely coordination of somatic mutagenesis and DNA repair in progenitor B (pro-B) cells. Here, we show that, in pro-B cells, the RNA-binding proteins T cell intracellular antigen 1 (TIA1) and TIA1-like protein (TIAL1) act redundantly to enable developmental progression. They are global splicing regulators that control the expression of hundreds of mRNAs, including those involved in DNA damage repair. Mechanistically, TIA1 and TIAL1 bind to 5' splice sites for exon definition, splicing, and expression of DNA damage sensors, such as Chek2 and Rif1. In their absence, pro-B cells show exacerbated DNA damage, altered P53 expression, and increased cell death. Our study uncovers the importance of tight regulation of RNA splicing by TIA1 and TIAL1 for the expression of integrative transcriptional programs that control DNA damage sensing and repair during B cell development.

| S-EPMC9794549 | biostudies-literature

CLIP-Seq of human to identify the global RNA binding sites for TIA1 and TIAL1 proteins

Project description:Individual nucleotide resolution UV-crosslinking and immunoprecipitation (iCLIP) and individual nucleotide resolution UV-crosslinking and affinity purification (iCLAP) were used to identify the global RNA binding sites for TIA1 and TIAL1 proteins. HeLa cells were UV crosslinked before lysing and digested with DNase and low concentration of RNase I. The protein-RNA complex were either immunoprecipitated with specific antibodies against either TIA1 or TIAL1 proteins and ligated to 3 prime adapter before separated by SDS-PAGE. For iCLAP, the His/Strep tagged proteins were overexpressed in HeLa cells, and the protein-RNA complexes were purified via Strep and His tag affinity purification. The proteins were digested by proteinase K and the RNA was reverse transcribed and self-circularised. The cDNA library was prepared by PCR with solexa primers compatible for high-throughput sequencing. This method allowed specific identify of crosslinked nucleotides, and genome-wide targets for TIA1 and TIAL1 not identified before. This method also allowed comparison between the 2 homologous proteins, whose binding sites and functions could be redundent. iCLAP provided an independent way to validate the binding sites identified by iCLIP, since no antibody was used in the iCLAP method.

2011-07-28 | E-MTAB-432 | biostudies-arrayexpress

iCLIP and iCLAP for TIA1 and TIAL1 proteins

Project description:iCLIP and iCLAP for TIA1 and TIAL1 proteins

| PRJEB2628 | ENA

The RNA binding proteins TIA1 and TIAL1 protect germinal centre responses from apoptosis

Project description:The RNA binding proteins TIA1 and TIAL1 protect germinal centre responses from apoptosis

| PRJNA985742 | ENA

The RNA binding proteins TIA1 and TIAL1 protect germinal centre responses from apoptosis [iCLIP]

Project description:The RNA binding proteins TIA1 and TIAL1 protect germinal centre responses from apoptosis [iCLIP]

| PRJNA986857 | ENA