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ChIP-Seq data of H3K79succ modification of mononuclear bodies in PHH cells infected with HBV

ABSTRACT: ChIP-Seq data of H3K79succ modification of mononuclear bodies in PHH cells infected with HBV

PROVIDER: PRJNA779727 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16921509_1.fastq.gz	Fastqsanger.gz
	SRR16921509_2.fastq.gz	Fastqsanger.gz
	SRR16921510_1.fastq.gz	Fastqsanger.gz
	SRR16921510_2.fastq.gz	Fastqsanger.gz

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ChIP-Seq data of H3K79succ modification of mononuclear bodies in PHH cells infected with HBV

Project description:Cell: HBV-infected PHH cells. Methods: PHH cells were infected with 2000 genome equivalents/cell of HBV particles in the presence of 4% PEG8000. Seven days after HBV infection, ChIP-Seq analysis of cccDNA in HBV-infected PHHs was carried out. HBV-infected PHH cells were digested with micrococcal nuclease and resulting mononucleosomes purified by sucrose gradient centrifugation. Nucleosomes were enriched with anti-H3K79succ antibodies by ChIP assay and the associated DNA contained both human and HBV DNA fragments was analyzed by deep sequencing. The HBV-specific reads in ChIP-Seq pilot experiments was quantified and normalized by the reads mapped to the human genome.

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