Ontology highlight
ABSTRACT:
PROVIDER: PRJNA781276 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR16970001_1.fastq.gz | Fastqsanger.gz | |||
| SRR16970001_2.fastq.gz | Fastqsanger.gz | |||
| SRR16970002_1.fastq.gz | Fastqsanger.gz | |||
| SRR16970002_2.fastq.gz | Fastqsanger.gz | |||
| SRR16970003_1.fastq.gz | Fastqsanger.gz |
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Project description:Background: Pear is one of the most important fruit crops worldwide. Anthocyanins and procyanidins (PAs) are important secondary metabolites that affect the appearance and nutritive quality of pear. However, few studies have focused on the molecular mechanism underlying anthocyanin and PA accumulation in pear. Results: We conducted metabolome and transcriptome analyses to identify candidate genes involved in anthocyanin and PA accumulation in young fruits of the pear cultivar ‘Clapp Favorite’ (CF) and its red mutation cultivar ‘Red Clapp Favorite’ (RCF). Gene–metabolite correlation analyses revealed a ‘core set’ of 20 genes that were strongly correlated with 10 anthocyanin and seven PA metabolites. Of these, PcGSTF12 was confirmed to be involved in anthocyanin and PA accumulation by complementation of the tt19-7 Arabidopsis mutant. Interestingly, PcGSTF12 was found to be responsible for the accumulation of procyanidin A3, but not petunidin 3, 5-diglucoside, opposite to the function of AtGSTs in Arabidopsis. Transformation with PcGSTF12 greatly promoted or repressed genes involved in anthocyanin and PA biosynthesis, regulation, and transport. Electrophoretic mobility shift and luciferase reporter assays confirmed positive regulation of PcGSTF12 by PcMYB114. Conclusion: These findings identify a core set of genes for anthocyanin and PA accumulation in pear. Of these, PcGSTF12 was confirmed to be involved in anthocyanin and PA accumulation. Our results also identified an important anthocyanin and PA regulation node comprising two core genes, PcGSTF12 and PcMYB114. These results provide novel insights into anthocyanin and PA accumulation in pear and represent a valuable data set to guide future functional studies and pear breeding.
2020-03-12 | GSE146798 | GEO
Project description:Organic acids and anthocyanins are the most important compounds for the flavor and nutritional quality of citrus fruit. In this study, a comparative transcriptome analysis was conducted to elucidate the genes and pathways involved in the accumulation of citrate and anthocyanins in postharvest citrus fruit using ‘Tarocco’ blood orange (TBO) and ‘Bingtangcheng’ sweet orange (BTSO). About 2397, 2677, 3067, 3131 and 2960 differentially expressed genes (DEGs) between two materials were identified at 0, 15, 30, 60, and 90 DAS (days after storage) respectively. A robust core set of 825 DEGs temporally associated with the citrate and anthocyanin accumulation throughout the storage period. Further function enrichment revealed the up-regulated DEGs in TBO were mainly related to UV protection, flavonoid biosynthesis process and transcription factors (TFs). And the down-regulated DEGs were mainly related to inositol biosynthesis process, carboxylic acid/organic acid transmembrane transport and transporters. Moreover, co-expression network and correlation analysis revealed that 23 differentially expressed TFs may be responsible for the simultaneously positively regulation of citrate and anthocyanin accumulation. And 19 differentially expressed transporters may be involved in citrate metabolism and negatively correlated with citrate content. These results suggesting that the high anthocyanis and citrate levels in TBO compared with BTSO were porbably attributed to the highly active of a group of core TFs and lowly active of transporters, which will accordingly provide novel insights into the molecular mechanisms underlying the difference accumulation of citrate and anthocyanin content in TBO and BTSO.
2023-04-27 | GSE212015 | GEO
Project description:Scope: Anthocyanins from diet were found to have therapeutic potential for various cancers including colorectal cancer (CRC). Given the diverse forms of anthocyanins, it is unclear whether different types of anthocyanins share common pathways in regulating intestinal cell function. This study aims to investigate the core gene pathways modulated by anthocyanins in intestinal cells, and to identify potential compounds with similar therapeutic effects.Methods and results: Three different forms anthocyanins were used to treat CRC cells at different concentrations and time points, and growth inhibition was observed in all anthocyanins, with varying patterns. RNA-seq analysis showed that the regulatory-pathways of anthocyanins with different structures were different, and the time factor had a greater effect. Time-series analysis of regulatory-genes shared by different concentrations and structures of anthocyanins was performed. By overlapping with public PPAR target gene set, a core gene set across three types of anthocyanins was identified and used in Connectivity Map (CMap) analysis to screen for compounds. The repositioned drug candidates included known anti-cancer drugs that have effects of transcriptome interventions similar to anthocyanins.Conclusion: These findings provide new insights into the mechanisms underlying the anti-CRC effects of anthocyanins and may facilitate the development of novel therapeutic agents for CRC.
2023-09-10 | GSE242673 | GEO
Project description:Purpose: High γ-aminobutyric acid (GABA)-producing Levilactobacillus brevis strain NPS-QW 145 along with Streptococcus thermophilus (one of the two starter bacteria used to make yogurt for its proteolytic activity) to enhance GABA production in milk. But a mechanistic understanding on how Levilactobacillus brevis cooperated with S. thermophilus to stimulate GABA production has been lacking. Method: Metatranscriptomic analyses combined with peptidomics were carried out to unravel the casein and lactose utilization patterns during milk fermentation with the co-culture. Results: We found particular peptides hydrolyzed by S. thermophilus 1275 were transported and biodegraded with peptidase in Lb. brevis 145 to meet the growth needs of the latter. In addition, amino acid synthesis and metabolism in Lb. brevis 145 were also activated to further support its growth. Glucose, as a result of lactose hydrolysis by S. thermophilus 1275, but not available lactose in milk, was outcompeted by Lb. brevis 145 as a main carbon source for glycolysis to produce ATP.In the stationary phase, under the acidic condition due to accumulation of lactic acid produced by S. thermophilus 1275, genes expression involved in pyridoxal phosphate (coenzyme of glutamic acid decarboxylase) metabolism and glutamic acid decarboxylase (Gad) in Lb. brevis 145 were induced for GABA production.
2020-09-16 | GSE157976 | GEO
Project description:Anthocyanins accumulation in the tea plant
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Project description:We found some intriguing proteins that provided new insights into molecular processes regulating sclereid accumulation in Korla pear pulp.
2020-04-27 | PXD018829 |
Project description:Genome-wide association studies provide insights into fruit biology of pear (Pyrus pyrifolia)
| PRJNA563813 | ENA